Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion ETD, Q Exactive
ORGANISM(S): Homo Sapiens (human) Escherichia Coli Staphylococcus Aureus
SUBMITTER: Anja Fux
LAB HEAD: Stephan A. Sieber
PROVIDER: PXD009224 | Pride | 2018-11-21
REPOSITORIES: Pride
Cell chemical biology 20181108 1
Detection of dynamic protein-protein interactions within complexes and networks remains a challenging task. Here, we show by the example of the proteolytic ClpXP complex the utility of combined chemical cross-linking and mass spectrometry (XL-MS) to map interactions within ClpP and ClpX as well as across the enigmatic ClpX hexamer-ClpP heptamer interface. A few hot-spot lysines located in signature loops in ClpX were shown to be in proximity to several structural regions of ClpP providing an ini ...[more]