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Deep proteomic analysis of murine erythroid differentiation

ABSTRACT: Erythropoiesis is a tightly controlled process. Although human and murine erythropoiesis are grossly similar, several aspects of these processes present significant differences. We have performed a label free quantification of proteins expressed during murine erythroid differentiation of primary cells with the aim to compare human and murine differentiations. Furthermore, we also analyzed the proteome of three murine cell lines, MEDEP, G1ER and Friend cells, to determine their similarities with the proteome of primary cells.

OTHER RELATED OMICS DATASETS IN: GSE120379

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Erythroid Lineage Cell, Cell Culture, Bone Marrow

SUBMITTER: Emilie-Fleur GAUTIER

LAB HEAD: Patrick MAYEUX

PROVIDER: PXD009384 | Pride | 2020-05-26

REPOSITORIES: Pride

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Publications

Comprehensive proteomic analysis of murine terminal erythroid differentiation.

Gautier Emilie-Fleur EF Leduc Marjorie M Ladli Meriem M Schulz Vincent P VP Lefèvre Carine C Boussaid Ismael I Fontenay Michaela M Lacombe Catherine C Verdier Frédérique F Guillonneau François F Hillyer Christopher D CD Mohandas Narla N Gallagher Patrick G PG Mayeux Patrick P

Blood advances 20200401 7

Murine-based cellular models have provided and continue to provide many useful insights into the fundamental mechanisms of erythropoiesis, as well as insights into the pathophysiology of inherited and acquired red cell disorders. Although detailed information on many aspects of these cell models is available, comprehensive proteomic data are lacking. This is a critical knowledge gap, as proteins are effectors of most biologic processes. To address this critical unmet need, proteomes of the murin ...[more]

PMID: 32282884

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