Proteomics

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Getting to the heart of the matter: Multispecies heart tissue proteome characterization

ABSTRACT: Structural birth defects are the leading cause of infant mortality in the US and Europe. Among these, congenital heart disease (CHD) is the most common. Historically, Xenopus, mouse, and pig have provided models for CHD. However, it remains unknown what proteins and pathways are conserved between these species and human. Furthermore, the proteome driving the differences between three-chambered (Xenopus) and four-chambered (mammalian) hearts is unknown. Comparative proteomics of heart tissue from species at different evolutionary points can reveal molecular processes underlying heart function. We examined heart tissue proteomes of Xenopus tropicalis, Xenopus laevis, Mus musculus, and Sus scrofa and assessed protein expression changes in the context of pathways and protein complexes, and enrichment of corresponding genes in human heart diseases.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Xenopus Laevis (african Clawed Frog) Xenopus Tropicalis Sus Scrofa Domesticus (domestic Pig) Mus Musculus (mouse)

TISSUE(S): Heart

SUBMITTER: Joel Federspiel  

LAB HEAD: Ileana M Cristea

PROVIDER: PXD009577 | Pride | 2019-08-01

REPOSITORIES: Pride

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Publications

Conservation and divergence of protein pathways in the vertebrate heart.

Federspiel Joel D JD   Tandon Panna P   Wilczewski Caralynn M CM   Wasson Lauren L   Herring Laura E LE   Venkatesh Samvida S SS   Cristea Ileana M IM   Conlon Frank L FL  

PLoS biology 20190906 9

Heart disease is the leading cause of death in the western world. Attaining a mechanistic understanding of human heart development and homeostasis and the molecular basis of associated disease states relies on the use of animal models. Here, we present the cardiac proteomes of 4 model vertebrates with dual circulatory systems: the pig (Sus scrofa), the mouse (Mus musculus), and 2 frogs (Xenopus laevis and Xenopus tropicalis). Determination of which proteins and protein pathways are conserved and  ...[more]

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