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Root proteome of ASPR1- deficient Arabidopsis thaliana compared to wt

ABSTRACT: Seedlings lacking the atypical aspartic protease encoded by the gene At2g03200 exhibited shorter primary roots and a pronounced reduction in the number of lateral roots. The protease was therefore name ATYPICAL ASPARTIC PROTEASE IN ROOTS 1 (ASPR1). This project compared the root proteome of a T-DNA insertion line lacking ASPR1 with wild type seedlings.

INSTRUMENT(S): maXis

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Root

SUBMITTER: Pitter Huesgen

LAB HEAD: Pitter Huesgen

PROVIDER: PXD009580 | Pride | 2019-02-25

REPOSITORIES: Pride

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An atypical aspartic protease modulates lateral root development in Arabidopsis thaliana.

Soares André A Niedermaier Stefan S Faro Rosário R Loos Andreas A Manadas Bruno B Faro Carlos C Huesgen Pitter F PF Cheung Alice Y AY Simões Isaura I

Journal of experimental botany 20190401 7

Few atypical aspartic proteases (APs) present in plants have been functionally studied to date despite having been implicated in developmental processes and stress responses. Here we characterize a novel atypical AP that we name Atypical Aspartic Protease in Roots 1 (ASPR1), denoting its expression in Arabidopsis roots. Recombinant ASPR1 produced by transient expression in Nicotiana benthamiana was active and displayed atypical properties, combining optimum acidic pH, partial sensitivity to peps ...[more]

PMID: 30778561

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