Mutation-specific and common phosphotyrosine signatures of KRAS G12D and G13D alleles

ABSTRACT: KRAS is one of the most frequently mutated genes across all cancer subtypes. Two of the most frequent oncogenic KRAS mutations observed in patients result in glycine to aspartic acid substitution at either codon 12 (G12D) or 13 (G13D). Although the biochemical differences between these two predominant mutations are not fully understood, distinct clinical features of the resulting tumors suggest involvement of disparate signaling mechanisms. When we compared the global and phosphotyrosine proteomic profiles of isogenic colorectal cancer cell lines bearing either G12D or G13D KRAS mutation, we observed both shared as well as unique signaling events induced by the two KRAS mutations.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

DISEASE(S): Colorectal Cancer

SUBMITTER: Akhilesh Pandey  

LAB HEAD: Akhilesh Pandey, M.D, Ph.D.

PROVIDER: PXD009843 | Pride | 2021-06-09

REPOSITORIES: Pride