Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap
ORGANISM(S): Homo Sapiens (human)
SUBMITTER: Christian Tüting
LAB HEAD: Elmar Wahle
PROVIDER: PXD010177 | Pride | 2020-05-26
REPOSITORIES: Pride
Schäfer Peter P Tüting Christian C Schönemann Lars L Kühn Uwe U Treiber Thomas T Treiber Nora N Ihling Christian C Graber Anne A Keller Walter W Meister Gunter G Sinz Andrea A Wahle Elmar E
RNA (New York, N.Y.) 20180823 12
Cleavage factor II (CF II) is a poorly characterized component of the multiprotein complex catalyzing 3' cleavage and polyadenylation of mammalian mRNA precursors. We have reconstituted CF II as a heterodimer of hPcf11 and hClp1. The heterodimer is active in partially reconstituted cleavage reactions, whereas hClp1 by itself is not. Pcf11 moderately stimulates the RNA 5' kinase activity of hClp1; the kinase activity is dispensable for RNA cleavage. CF II binds RNA with nanomolar affinity. Bindin ...[more]