Dataset Information

ITRAQ-based quantitative proteomic analysis reveals proteomic changes in leaves of mulberry in response to drought stress

ABSTRACT: Mulberry (Morus alba) is a fast-growing perennial woody plant with a long cultivation history in China and a distribution throughout the country. To date, the molecular mechanism of mulberry response to abiotic stress is little known. In the present study, proteomics data were collected from the leaves of the mulberry cultivar Neo-Ichinose (Japan) under drought stress and analyzed. In total, 2871 proteins were quantified, among which 267 proteins were differentially expressed. A Gene Ontology enrichment analysis indicated that the molecular functions of the up-regulated proteins were mainly related to glutathione peroxidase activity, hydrolase activity, oxidoreductase activity, peroxidase activity and antioxidant activity. Protein domain enrichment analysis showed that the most important protein domains were associated with glutathione peroxidase, glycoside hydrolase, and chaperones. Additionally, a functional enrichment analysis demonstrated that the main metabolic pathways of mulberry seedlings in response to drought stress were arachidonic acid metabolism and glutathione metabolism, which were up-regulated, and chlorophyll and porphyrin metabolism and glycerol metabolism, which were down-regulated. Additionally, many proteins related to osmotic adjustment and stress signal transduction were evoked by drought. Taken together, these results suggested that redox control and osmotic adjustment mediated by multiple signaling pathways dominated the drought response of mulberry and that antioxidant proteins and enzymes, especially glutathione peroxidase, played vital roles in the redox rebalance of mulberry under stress.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Morus Alba

TISSUE(S): Leaf

SUBMITTER: suzhen huang

LAB HEAD: Yong Hua Qian

PROVIDER: PXD010227 | Pride | 2022-10-13

REPOSITORIES: Pride

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Publications

Quantitative Proteomics and Functional Characterization Reveal That Glutathione Peroxidases Act as Important Antioxidant Regulators in Mulberry Response to Drought Stress.

Zhang Minjuan M Li Wenqiang W Li Shuaijun S Gao Junru J Gan Tiantian T Li Qinying Q Bao Lijun L Jiao Feng F Su Chao C Qian Yonghua Y

Plants (Basel, Switzerland) 20220908 18

Mulberry (<i>Morus alba</i> L.) has been an economically important food crop for the domesticated silkworm, <i>Bombyx mori</i>, in China for more than 5000 years. However, little is known about the mechanism underlying mulberry response to environmental stress. In this study, quantitative proteomics was applied to elucidate the molecular mechanism of drought response in mulberry. A total of 604 differentially expressed proteins (DEPs) were identified via LC-MS/MS. The proteomic profiles associat ...[more]

PMID: 36145752

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Dataset Information

ITRAQ-based quantitative proteomic analysis reveals proteomic changes in leaves of mulberry in response to drought stress

Publications

Quantitative Proteomics and Functional Characterization Reveal That Glutathione Peroxidases Act as Important Antioxidant Regulators in Mulberry Response to Drought Stress.

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