Dataset Information

Analysis of Erysiphe pisi proteome

ABSTRACT: Erysiphe pisi causes powdery mildew disease in garden pea. It is a biotrophic ascomycete member necessitating a living host for its survival. An attempt to identify the global proteome of E. pisi pathogen is made using a sensitive and reliable nano LC-MS/MS approach. The protein profiling of two isolates of E. pisi; Ep01 and Ep02 varying for virulence upon testing on a commercial cultivar, Arkel led to the detection of a total of 211 and 214 distinct proteins in Ep01 and Ep03 isolates respectively. In addition, a total of 203 and 207proteins from Ep01 and Ep03 isolates respectively were found to be hypothetical or proteins with not yet predicted functions based on GO (biological process). The protein accessions detected in these isolates were categorized into functional protein classes with some of the identified proteins reported to be involved in pathogenesis or virulence. The proteins belonging to the functional classes like stress related, signal transduction and secondary metabolite formation might be involved in virulence and pathogenesis. The proteome proposed in this study would serve as a reference proteome to facilitate the understanding of the functional aspects of an obligate biotrophic fungal pathogen.

INSTRUMENT(S): LTQ Orbitap Discovery

ORGANISM(S): Erysiphe Pisi

TISSUE(S): Fungal Asexual Spore, Mycelium

DISEASE(S): Fungal Infectious Disease

SUBMITTER: Ragiba Makandar

LAB HEAD: Prof. Ragiba Makandar

PROVIDER: PXD010238 | Pride | 2019-04-02

REPOSITORIES: Pride

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Publications

Shotgun proteomics provides an insight into pathogenesis-related proteins using anamorphic stage of the biotroph, Erysiphe pisi pathogen of garden pea.

Bheri Malathi M M Bhosle Sheetal S Makandar Ragiba R

Microbiological research 20190212

E. pisi is an ascomycete member causing powdery mildew disease of garden pea. It is a biotrophic pathogen, requiring a living host for its survival. Our understanding of molecular mechanisms underlying its pathogenesis is limited. The identification of proteins expressed in the pathogen is required to gain an insight into the functional mechanisms of an obligate biotrophic fungal pathogen. In this study, the proteome of the anamorphic stage of E. pisi pathogen has been elucidated through the nan ...[more]

PMID: 30928027

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Dataset Information

Analysis of Erysiphe pisi proteome

Publications

Shotgun proteomics provides an insight into pathogenesis-related proteins using anamorphic stage of the biotroph, Erysiphe pisi pathogen of garden pea.

Similar Datasets

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