Analysis of intact mannose-6-phosphate glycopeptides

ABSTRACT: We have repurposed standard phosphoproteomics workflow based on Fe3+IMAC for enrichment ofmannose-6-phosphate modified glycopeptides. This worklfow was used to profile lysosomal acid hydrolases in HeLa and CHO cell lines. We have combined this approach with CRISPR/Cas9 KO of acid phosphatases 2 and 5 responsible for dephosphorylation of mannose-6-phosphate glycopeptides in the lysosome. This combined approach enabled significantly deeper coverage of CHO mannose-6-phosphate glycoproteome.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human) Cricetulus Griseus (chinese Hamster) (cricetulus Barabensis Griseus)

TISSUE(S): Cell Suspension Culture, Hela Cell

SUBMITTER: TOMISLAV CAVAL  

LAB HEAD: Albert J R Heck

PROVIDER: PXD010333 | Pride | 2018-12-18

REPOSITORIES: Pride