Analysis of the fission yeast meiotic proteome reveals a global switch from anabolic to catabolic processes and extensive post-transcriptional regulation.

ABSTRACT: Progression through meiosis in Schizosaccharomyces pombe is regulated by stage-specific gene expression and translation, changes in RNA stability, expression of anti-sense transcripts and also requires stage-specific, targetted proteolysis of regulatory proteins. We have used SILAC labelling to examine the relative levels of proteins in S. pombe as diploid cells undergo meiosis. We found that the relative level of 880 proteins changes at least two-fold at some stage of meiosis. Most of these proteins either increase or decrease in level during the meiotic divisions, while some show transient peaks of expression. The most notable changes are in the proteostasis network, which shows a significant increase in components involved in protein turnover concomitant to a decrease in proteins involved in ribosome biogenesis. There was also an increase in ESCRT III protein levels; biological analysis reveals a role for some ESCRT III components in chromosome segregation and spore formation. Correlation with previous studies of gene expression and ribosome occupancy through meiosis reveals that changes in steady state protein levels are mainly regulated post-transcriptionally.

INSTRUMENT(S):

ORGANISM(S): Schizosaccharomyces Pombe

SUBMITTER: Romain Hamelin  

LAB HEAD: Viesturs Simanis

PROVIDER: PXD010438 | Pride | 2019-02-15

REPOSITORIES: Pride