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Proteomic analysis of plasmodesmata from Populus cell suspension cultures

ABSTRACT: In this project, we have investigated the proteome of the plasmodesmata (PD) enriched membrane fraction from poplar cell suspension cultures and quantitatively compared its protein composition with other membrane fractions. We build on the previously published literature on the PD proteome by using semiquantitative methods and, for the first time, demonstrate in vitro callose synthase activity from Populus PD proteins. To our knowledge, this study is the first proteomic investigation of the PD performed on a tree species.

INSTRUMENT(S): Q-Tof ultima

ORGANISM(S): Populus Trichocarpa (western Balsam Poplar) (populus Balsamifera Subsp. Trichocarpa)

TISSUE(S): Plant Cell, Primary Cell

SUBMITTER: Vaibhav Srivastava

LAB HEAD: Vaibhav Srivastava

PROVIDER: PXD010692 | Pride | 2018-12-31

REPOSITORIES: Pride

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Proteomic Analysis of Plasmodesmata From <i>Populus</i> Cell Suspension Cultures in Relation With Callose Biosynthesis.

Leijon Felicia F Melzer Michael M Zhou Qi Q Srivastava Vaibhav V Bulone Vincent V

Frontiers in plant science 20181119

Plasmodesmata are channels that link adjacent cells in plant tissues through which molecular exchanges take place. They are involved in multiple processes vital to plant cells, such as responses to hormonal signaling or environmental challenges including osmotic stress, wounding and pathogen attack. Despite the importance of plasmodesmata, their proteome is not well-defined. Here, we have isolated fractions enriched in plasmodesmata from cell suspension cultures of <i>Populus trichocarpa</i> and ...[more]

PMID: 30510561

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