Dataset Information

Giant panda (Ailuropoda melanoleuca) gut microbiomes degrade bamboo in distinct patterns: heterofermentation of green leaves to ethanol, lactate and acetate, and homofermentation of yellow pith to lactate

ABSTRACT: The iconic giant panda is an endangered species known worldwide for its peculiar dietary habits. While retaining the digestive system of a carnivore, the giant panda successfully moved into a diet almost exclusively based on bamboo. Digestion of lignocellulose is believed to be conducted solely by its gut microbiome, provided that no lignocellulose-degrading enzyme was found in the giant panda’s genome. Many reports focused on which lignocellulose component feeds the giant panda, while little effort was made to link the products of bamboo fermentation to the panda’s dietary choices. In the present study, fermentation of either green leaves or yellow pith was conducted in the laboratory using gut microbiomes derived from either green or yellow stools, respectively. Green leaves were fermented to ethanol, lactate and acetate, while yellow pith to lactate resembling, respectively, hetero/homo-fermentation patterns. Several microbial pathways (assessed by metaproteomics) related to hemicellulose rather than cellulose degradation. However, alpha-amylases (E.C. 3.2.1.1) from the giant panda itself were the most predominant enzyme (up to 60% of all metaproteins), indicating that they have a primary role in bamboo digestion. The distinct fermentation profiles resulting from digestion of selected portions of bamboo may be part of the feeding strategy of giant pandas.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Escherichia Coli Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Bile

SUBMITTER: Robert Heyer

LAB HEAD: Dirk Benndorf

PROVIDER: PXD010872 | Pride | 2020-03-02

REPOSITORIES: Pride

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Publications

Substrate-Dependent Fermentation of Bamboo in Giant Panda Gut Microbiomes: Leaf Primarily to Ethanol and Pith to Lactate.

Scoma Alberto A Khor Way Cern WC Coma Marta M Heyer Robert R Props Ruben R Schoelynck Jonas J Bouts Tim T Benndorf Dirk D Li Desheng D Zhang Hemin H Rabaey Korneel K

Frontiers in microbiology 20200331

The giant panda is known worldwide for having successfully moved to a diet almost exclusively based on bamboo. Provided that no lignocellulose-degrading enzyme was detected in panda's genome, bamboo digestion is believed to depend on its gut microbiome. However, pandas retain the digestive system of a carnivore, with retention times of maximum 12 h. Cultivation of their unique gut microbiome under controlled laboratory conditions may be a valid tool to understand giant pandas' dietary habits, an ...[more]

PMID: 32300339

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Dataset Information

Giant panda (Ailuropoda melanoleuca) gut microbiomes degrade bamboo in distinct patterns: heterofermentation of green leaves to ethanol, lactate and acetate, and homofermentation of yellow pith to lactate

Publications

Substrate-Dependent Fermentation of Bamboo in Giant Panda Gut Microbiomes: Leaf Primarily to Ethanol and Pith to Lactate.

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