Dataset Information

A proteomic and functional analysis of the metabolic adaptive response to iron during the filamentation process of the pathogen Candida albicans.

ABSTRACT: Candia albicans is an opportunist pathogen responsible for a large spectrum of infections, from superficial mycosis to systemic diseases called candidiasis. Its ability to grow in various morphological forms, such as yeast and filamentous hyphae, contributes to its survival in the diverse microenvironments it encounters in the host, differing in key micronutrients and iron contents. In C. albicans, iron uptake has been associated with virulence and the pathogen has developed elaborated strategies to acquire iron from the host. In this study, we performed a global proteomic analysis of the changes of protein abundances in response to iron, and correlated these data with morphological and functional studies of the main metabolic pathways of the pathogen, both in the yeast and filamentous forms. Our results show that C. albicans yeast cells have an extraordinary capability to adapt to both iron excess and iron deficiency. Moreover, changes of iron content in the growth medium are associated with a metabolic remodeling affecting intracellular glutathione levels and reduced thiol groups, but also enzymes of the TCA cycle, the pentose phosphate pathway or the mitochondrial respiratory chain. Our data indicate that iron-deficiency stimulates the bioenergetics pathways, whereas iron excess corresponds to more biosynthetic needs, especially in the filamentous forms. Altogether, our results emphasize the influence of the environment of C. albicans cells on the filamentation process, and show evidence for the differences between the yeast and filamentous forms, which seem to adapt more easily in response to a large set of iron conditions.

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Candida Albicans (yeast)

SUBMITTER: Camille GARCIA

LAB HEAD: Jean-Michel Camadro

PROVIDER: PXD010881 | Pride | 2020-05-26

REPOSITORIES: Pride

ACCESS DATA

Publications

The adaptive response to iron involves changes in energetic strategies in the pathogen Candida albicans.

Duval Celia C Macabiou Carole C Garcia Camille C Lesuisse Emmanuel E Camadro Jean-Michel JM Auchère Françoise F

MicrobiologyOpen 20191201 2

Candida albicans is an opportunist pathogen responsible for a large spectrum of infections, from superficial mycosis to systemic diseases known as candidiasis. Its ability to grow in different morphological forms, such as yeasts or filamentous hyphae, contributes to its survival in diverse microenvironments. Iron uptake has been associated with virulence, and C. albicans has developed elaborate strategies for acquiring iron from its host. In this work, we analyze the metabolic changes in respons ...[more]

PMID: 31788966

Similar Datasets

Project description:The interaction between fungal pathogen and host during infection is a complex and dynamic process. To resolve this, we chose the zebrafish model organism as the host to study C. albicans infection via systems biology approach. Transcriptome microarray data and histological analysis of surviving fish were sampled at different post-infection time points. The dynamic variations of significant genes expression profiles in C. albicans and zebrafish were concurrently analyzed by principal component analysis (PCA). The PCA results clearly indicated that the infection of C. albicans can be divided into three phases that include adhesion, invasion and damage phases. The results were highly consistent with subsequent histological analysis. Furthermore, we found the primary ontology function of genes with significant variations in both C. albicans and zebrafish is iron related. Most of the iron related genes in C. albicans were over-expressed in late stage, while most of the iron related genes in zebrafish were suppressed at the same phase of infection. It suggested that the iron homeostasis function of the host was shut-down when massive hemorrhage in zebrafish occurred during later stages of infection. At the same time, the iron scavenging function of C. albicans was activated. This implied the competition for iron is an important issue between the host and the fungal pathogen during infection. When we administered excess iron into the microenvironment of infection site, the infection process was significantly delayed. That indicated the virulence of C. albicans is correlated with its protein-bond iron scavenging strategies. Our finings not only provided dynamic mechanistic views of the iron competition but also highlighted the potential regulatory schemes in fungal pathogenesis. Each fish was intraperitoneally injected with C. albicans cells and these infected fish were collected at 0.5, 1, 2, 4, 6, 8, 12, 16, 18 hpi. 0.625M-NM-<g of Cy3 cRNA for C. albicans array and 1.65 M-NM-<g of Cy3 cRNA for zebrafish array was fragmented to an average size of about 50-100 nucleotides by incubation with fragmentation buffer at 60M-BM-0C for 30 minutes. Each time points contain three biological repeat. For C. albicans array, each biological repeat has two technical replicates.

Dataset Information

A proteomic and functional analysis of the metabolic adaptive response to iron during the filamentation process of the pathogen Candida albicans.

Publications

The adaptive response to iron involves changes in energetic strategies in the pathogen Candida albicans.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure