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Porcine breast extracellular matrix hydrogel for spatial tissue culture

ABSTRACT: Porcine mammary fatty tissues represent an abundant source of biomaterial for generation of breast-specific extracellular matrix (ECM). Here we report the extraction of total ECM proteins from pig breast fatty tissues, the fabrication of hydrogel scaffolds from the extracted ECM proteins, the structural properties of the scaffolds, and the applications of the hydrogel in human mammary epithelial cell spatial cultures for cell surface receptor expression, metabolomics characterization, acini formation, proliferation, migration between different scaffolding compartments, and in vivo tumor formation. This model system provides an additional option for studying human breast diseases such as breast cancer.

INSTRUMENT(S): Bruker Daltonics solarix series

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Jing Wang

LAB HEAD: Weimin Li

PROVIDER: PXD010960 | Pride | 2019-11-12

REPOSITORIES: Pride

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Porcine Breast Extracellular Matrix Hydrogel for Spatial Tissue Culture.

Rijal Girdhari G Wang Jing J Yu Ilhan I Gang David R DR Chen Roland K RK Li Weimin W

International journal of molecular sciences 20180925 10

Porcine mammary fatty tissues represent an abundant source of natural biomaterial for generation of breast-specific extracellular matrix (ECM). Here we report the extraction of total ECM proteins from pig breast fatty tissues, the fabrication of hydrogel and porous scaffolds from the extracted ECM proteins, the structural properties of the scaffolds (tissue matrix scaffold, TMS), and the applications of the hydrogel in human mammary epithelial cell spatial cultures for cell surface receptor expr ...[more]

PMID: 30257480

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