Proteomics

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Phosphoproteomic profiling reveals a defined genetic program for osteoblastic lineage commitment of human bone marrow derived stromal stem cells

ABSTRACT: Bone marrow-derived mesenchymal stem cells (MSCs) differentiate into osteoblasts upon induction by signals present in their niche. As the global signaling cascades involved in the early phases of MSCs osteoblast (OB) differentiation are not well-defined, we employed quantitative mass spectrometry (SILAC based) to delineate changes in human MSCs proteome and phosphoproteome during the first 24 hours of their OB lineage commitment. The temporal profiles of 6,252 proteins and 15,059 phosphorylation sites suggested at least two distinct signaling waves: one peaking within 30 to 60 min after induction and a second upsurge after 24 hours

INSTRUMENT(S): LTQ Orbitrap, LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Stem Cell, Cell Culture

SUBMITTER: Inigo Barrio  

LAB HEAD: Blagoy Blagoev

PROVIDER: PXD011001 | Pride | 2021-09-08

REPOSITORIES: Pride

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Phosphoproteomic profiling reveals a defined genetic program for osteoblastic lineage commitment of human bone marrow-derived stromal stem cells.

Barrio-Hernandez Inigo I   Jafari Abbas A   Rigbolt Kristoffer T G KTG   Hallenborg Philip P   Sanchez-Quiles Virginia V   Skovrind Ida I   Akimov Vyacheslav V   Kratchmarova Irina I   Dengjel Joern J   Kassem Moustapha M   Blagoev Blagoy B  

Genome research 20191212 1

Bone marrow-derived mesenchymal stem cells (MSCs) differentiate into osteoblasts upon stimulation by signals present in their niche. Because the global signaling cascades involved in the early phases of MSCs osteoblast (OB) differentiation are not well-defined, we used quantitative mass spectrometry to delineate changes in human MSCs proteome and phosphoproteome during the first 24 h of their OB lineage commitment. The temporal profiles of 6252 proteins and 15,059 phosphorylation sites suggested  ...[more]

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