Proteomics

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Identification of proteins associated with Kv1.2 potassium channels.

ABSTRACT: Kv1.2 is a prominent voltage-gated potassium channel that influences action potential generation and propagation in the central nervous system. We explored multi-protein complexes containing Kv1.2 using mass spectrometry of immunoprecipitated samples. Proteins identified as candidate interactors were prioritized based on abundance, and were screened for functional effects on Kv1.2 channels using electrophysiology.

INSTRUMENT(S): Bruker Daltonics instrument model

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Embryonic Kidney Cell Line

SUBMITTER: Jenny Moon  

LAB HEAD: Harley Takatsuna Kurata

PROVIDER: PXD011010 | Pride | 2018-11-04

REPOSITORIES: Pride

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Publications

Slc7a5 regulates Kv1.2 channels and modifies functional outcomes of epilepsy-linked channel mutations.

Baronas Victoria A VA   Yang Runying Y RY   Morales Luis Carlos LC   Sipione Simonetta S   Kurata Harley T HT  

Nature communications 20181024 1

Kv1.2 is a prominent voltage-gated potassium channel that influences action potential generation and propagation in the central nervous system. We explored multi-protein complexes containing Kv1.2 using mass spectrometry followed by screening for effects on Kv1.2. We report that Slc7a5, a neutral amino acid transporter, has a profound impact on Kv1.2. Co-expression with Slc7a5 reduces total Kv1.2 protein, and dramatically hyperpolarizes the voltage-dependence of activation by -47 mV. These effec  ...[more]

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