Dataset Information
LOPIT-DC subcellular map of U-2 OS cells
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion Lumos
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Permanent Cell Line Cell, Cell Culture
DISEASE(S): Bone Osteosarcoma
SUBMITTER: 
Aikaterini Geladaki
LAB HEAD: Kathryn Lilley
PROVIDER: PXD011254 | Pride | 2019-01-23
REPOSITORIES: Pride
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| DC_REP1_F1.dat | Other | |||
| DC_REP1_F1.raw | Raw | |||
| DC_REP1_F10.dat | Other | |||
| DC_REP1_F10.raw | Raw | |||
| DC_REP1_F11.dat | Other |
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Publications
Combining LOPIT with differential ultracentrifugation for high-resolution spatial proteomics.
Nature communications 20190118 1
The study of protein localisation has greatly benefited from high-throughput methods utilising cellular fractionation and proteomic profiling. Hyperplexed Localisation of Organelle Proteins by Isotope Tagging (hyperLOPIT) is a well-established method in this area. It achieves high-resolution separation of organelles and subcellular compartments but is relatively time- and resource-intensive. As a simpler alternative, we here develop Localisation of Organelle Proteins by Isotope Tagging after Dif ...[more]
