Proteomics

Dataset Information

6

High-density chemical cross-linking for modeling protein interactions


ABSTRACT: We combine several orthogonal cross-linking chemistries as well as improvements in search algorithms, statistical analysis and computational cost to achieve coverage of one unique cross-linked position pair for every 7 amino acids at a 1% false discovery rate. This is accomplished without any peptide-level fractionation, enrichment, or isotopic labeling. We apply our methods to model the complex between a carbonic anhydrase (CA) and its protein inhibitor, as well as the yeast proteasome

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Escherichia coli   Saccharomyces cerevisiae   Homo sapiens   Bos taurus  

TISSUE(S): Tissue Not Applicable To Dataset

DISEASE(S): Not Available

SUBMITTER: Julian Mintseris  

LAB HEAD: Steven P. Gygi

PROVIDER: PXD011296 | Pride | 2019-12-06

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
REV9mix.fasta Fasta
REVPrtsmRpn11.fasta Fasta
REVmICA.fasta Fasta
t02630_150120_9mix_3hr_EDC.raw Raw
t02630_150120_9mix_3hr_EDC.tgz Other
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