ABSTRACT: carbonic anhydrase (1 or 2) simple or double mutants-Comparative transcriptome analysis between carbonic anhydrase mutants and wild type Arabidopsis
Project description:cea05-01_carbonic-anhydrase - carbonic anhydrase (1 or 2) simple or double mutants - Identification of genes differentially expressed in leaves of single CA1 and CA2 T-DNA insertional mutants and in the corresponding double mutant vs wild type - CA1 (At3g01500) and CA2 (At5g14740) T-DNA insertional mutant lines, the double (CA1+CA2) mutant and wild type Arabidopsis seeds were sown in soil in a phytotron. Leaves were harvested 40 days later for RNA extraction
Project description:cea05-01_carbonic-anhydrase - carbonic anhydrase (1 or 2) simple or double mutants - Identification of genes differentially expressed in leaves of single CA1 and CA2 T-DNA insertional mutants and in the corresponding double mutant vs wild type - CA1 (At3g01500) and CA2 (At5g14740) T-DNA insertional mutant lines, the double (CA1+CA2) mutant and wild type Arabidopsis seeds were sown in soil in a phytotron. Leaves were harvested 40 days later for RNA extraction 6 dye-swap - gene knock out
Project description:Photosynthetic efficiency and stress tolerance in plants are regulated by complex interactions between photoprotective mechanisms and carbon metabolism. The photosystem II subunit PsbS plays a central role in non-photochemical quenching, while β-carbonic anhydrases contribute to CO₂ conversion and pH homeostasis in chloroplasts. To investigate the potential crosstalk between these components, we performed RNA-seq analysis of Arabidopsis thaliana lines overexpressing PsbS alone or in combination with β-carbonic anhydrase genes (βCA1 and βCA2). Wild-type (Col-0), the npq4 mutant, and transgenic overexpression lines were grown under controlled conditions and treated either with water or bicarbonate fertilization. Transcriptome profiling was performed using Illumina NovaSeq sequencing of poly(A)+ RNA libraries prepared from total RNA. The dataset contains three biological replicates for each genotype and treatment combination. These data provide insight into transcriptional responses associated with altered PsbS and β-carbonic anhydrase activity and bicarbonate-induced signaling, and can be used to explore regulatory networks linking photoprotection, carbon metabolism, and stress responses in plants.
Project description:geLC-MS/MS analysiswas performed to identify proteins that are present in apoplastic fluid isolated from rosette leaves of 8-week-old wild type and carbonic anhydrase (ca1ca4) mutant Arabidopsis plants.
Project description:In Nannochloropsis oceanica IMET1, transcript knockdown of a cytosolic carbonic anhydrase (CA2; g2018) specifically inhibited by HC resulted in ~45%, ~30% and ~40% elevation of photosynthetic oxygen evolution rate, growth rate and biomass accumulation rate under high CO2 (5% ), respectively. This CA2-knockdown mutant is demonated as M2. To probe mechanistic links underlying the mutant (M2; RNAi-knockdown line of carbonic anhydrase (CA2)) phenotypes, temporal transcriptomic profiles are compared between RNAi-knockdown line of carbonic anhydrase (CA2) and WT, at 12 h and 24h under high CO2 (5%).
Project description:geLC-MS/MS analysis was performed to identify proteins that are present in apoplastic fluid isolated from rosette leaves of 8-week-old wild type and carbonic anhydrase (ca1ca4) mutant Arabidopsis. Plants were grown in either low (150 ppm) or high (500 ppm) CO2.
2014-08-22 | PXD000693 | Pride
Project description:Maize carbonic anhydrase mutants at high and low CO2
Project description:Carbonic anhydrase IX (CA 9) is a transmembrane isoform of carbonic anhydrase (CA) that contributes to an acidification of tumor microenvironment. The expression of CA 9 in cervical tumors was shown to be strongly involved in high incidence of metastasis and poor prognosis. To search for the key regulators of metastasis related to ectopic expression of CA 9, we investigated differentially expressed gene profiles in CA 9- transfected cervix carcinoma cell line C33-A (CF) compared with mock-transfected (CP) cell line, using Affymetrix Human Genome U133 Plus 2.0 Array.
