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Secretomics of the Staphylococcus aureus Type VII protein secretion system

ABSTRACT: The type VII protein secretion system (T7SS) is conserved across Staphylococcus aureus strains and plays important roles in virulence and interbacterial competition. To date only one T7SS substrate protein, encoded in a subset of strains, has been functionally characterized. Here, using an unbiased proteomic approach, we identify TspA as a further T7SS substrate.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Staphylococcus Aureus

SUBMITTER: Matthias Trost

LAB HEAD: Matthias Trost

PROVIDER: PXD011358 | Pride | 2020-09-28

REPOSITORIES: Pride

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A membrane-depolarizing toxin substrate of the Staphylococcus aureus type VII secretion system mediates intraspecies competition.

Ulhuq Fatima R FR Gomes Margarida C MC Duggan Gina M GM Guo Manman M Mendonca Chriselle C Buchanan Grant G Chalmers James D JD Cao Zhenping Z Kneuper Holger H Murdoch Sarah S Thomson Sarah S Strahl Henrik H Trost Matthias M Mostowy Serge S Palmer Tracy T

Proceedings of the National Academy of Sciences of the United States of America 20200807 34

The type VII protein secretion system (T7SS) is conserved across Staphylococcus aureus strains and plays important roles in virulence and interbacterial competition. To date, only one T7SS substrate protein, encoded in a subset of S. aureus genomes, has been functionally characterized. Here, using an unbiased proteomic approach, we identify TspA as a further T7SS substrate. TspA is encoded distantly from the T7SS gene cluster and is found across all S. aureus strains as well ...[more]

PMID: 32769205

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