QToF Cross-linking Optimisation
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Synapt MS
ORGANISM(S): Bos Taurus (bovine)
TISSUE(S): Blood Serum
SUBMITTER: 
Juliette James
LAB HEAD: Konstantinos Thalassinos
PROVIDER: PXD011704 | Pride | 2019-11-12
REPOSITORIES: Pride
Publications
Optimization Workflow for the Analysis of Cross-Linked Peptides Using a Quadrupole Time-of-Flight Mass Spectrometer.
James Juliette M B JMB Cryar Adam A Thalassinos Konstantinos K
Analytical chemistry 20190123 3
Cross-linking mass spectrometry is an emerging structural biology technique. Almost exclusively, the analyzer of choice for such an experiment has been the Orbitrap. We present an optimized protocol for the use of a Synapt G2-Si for the analysis of cross-linked peptides. We first tested six different energy ramps and analyzed the fragmentation behavior of cross-linked peptides identified by xQuest. By combining the most successful energy ramps, cross-link yield can be increased by up to 40%. Whe ...[more]
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