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AP-MS analysis of FLAG-tagged Murine norovirus (NS1/2 and NS4)

ABSTRACT: Norovirus replication is accomplished by the use of a number of viral proteins derived from a long polyprotein. These components are named from NS1/2 to NS7. Having previously determined that the incorporation of FLAG epitope tags into certain positions in the norovirus genome was tolerated (Thorne et. al. 2012 J. Virol)

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Ssrna Viruses Mus Musculus (mouse)

TISSUE(S): Cell Culture, Macrophage

SUBMITTER: Edward Emmott

LAB HEAD: Ian Goodfellow

PROVIDER: PXD011779 | Pride | 2019-09-09

REPOSITORIES: Pride

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Noroviruses subvert the core stress granule component G3BP1 to promote viral VPg-dependent translation.

Hosmillo Myra M Lu Jia J McAllaster Michael R MR Eaglesham James B JB Wang Xinjie X Emmott Edward E Domingues Patricia P Chaudhry Yasmin Y Fitzmaurice Tim J TJ Tung Matthew Kh MK Panas Marc Dominik MD McInerney Gerald G Locker Nicolas N Wilen Craig B CB Goodfellow Ian G IG

eLife 20190812

Knowledge of the host factors required for norovirus replication has been hindered by the challenges associated with culturing human noroviruses. We have combined proteomic analysis of the viral translation and replication complexes with a CRISPR screen, to identify host factors required for norovirus infection. The core stress granule component G3BP1 was identified as a host factor essential for efficient human and murine norovirus infection, demonstrating a conserved function across the <i>Nor ...[more]

PMID: 31403400

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