Wnt-modulated hiPSC-derived insulin-producing cells, adult human islet proteome

ABSTRACT: We aimed to assess whether Wnt-modulation could contribute to mature hiPSC-derived insulin-producing cells in vitro. Building our hypothesis on our previous findings of Wnt activation in immature hiPSC-derived insulin-producing cells compared to adult human islets and with recent data reporting a link between Wnt/PCP and in vitro beta-cell maturation. In this study we stimulated hiPSC-derived insulin-producing cells with syntetic proteins including WNT3A, WNT4, WNT5A and WNT5B as well as inhibiting endogeneous Wnt signaling with Tankyrase inhibitor G007-LK.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Islet Of Langerhans, Cell Culture

SUBMITTER: Heidrun Vethe  

LAB HEAD: Helge Ræder

PROVIDER: PXD012081 | Pride | 2019-11-13

REPOSITORIES: Pride