Proteomics

Dataset Information

1

Wnt-modulated hiPSC-derived insulin-producing cells, adult human islet proteome


ABSTRACT: We aimed to assess whether Wnt-modulation could contribute to mature hiPSC-derived insulin-producing cells in vitro. Building our hypothesis on our previous findings of Wnt activation in immature hiPSC-derived insulin-producing cells compared to adult human islets and with recent data reporting a link between Wnt/PCP and in vitro beta-cell maturation. In this study we stimulated hiPSC-derived insulin-producing cells with syntetic proteins including WNT3A, WNT4, WNT5A and WNT5B as well as inhibiting endogeneous Wnt signaling with Tankyrase inhibitor G007-LK.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo sapiens  

TISSUE(S): Cell Culture

DISEASE(S): Not Available

SUBMITTER: Heidrun Vethe  

LAB HEAD: Helge Ræder

PROVIDER: PXD012081 | Pride | 2019-11-13

REPOSITORIES: pride

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Publications

The Effect of Wnt Pathway Modulators on Human iPSC-Derived Pancreatic Beta Cell Maturation.

Vethe Heidrun H   Ghila Luiza L   Berle Magnus M   Hoareau Laurence L   Haaland Øystein A ØA   Scholz Hanne H   Paulo Joao A JA   Chera Simona S   Ræder Helge H  

Frontiers in endocrinology 20190508


Current published protocols for targeted differentiation of human stem cells toward pancreatic β-cells fail to deliver sufficiently mature cells with functional properties comparable to human islet β-cells. We aimed to assess whether Wnt-modulation could promote the final protocol stages of β-cell maturation, building our hypothesis on our previous findings of Wnt activation in immature hiPSC-derived stage 7 (S7) cells compared to adult human islets and with recent data reporting a link between  ...[more]

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