Activation of Wnt signaling pathways in murine B16-F1 melanoma cells

ABSTRACT: Genome-wide transcriptional profiling using microarrays was used to compare gene regulation in B16 murine melanoma cells that were: 1) stably transduced with Wnt1-iresGFP; 2) stably transduced with Wnt3A-iresGFP; 3) stably transduced with Wnt5A-iresGFP; and 4) stably transduced with GFP and treated for 72 hours with 10 mM lithium chloride, a pharmacologic activator of canonical Wnt/beta-catenin signaling. Cells were sorted by fluorescence-activated cell sorting (FACS) to obtain populations with relatively equivalent levels of GFP expression. Biologic triplicates were used for each condition, and compared in two-channel hybridization to control RNA obtained from B16 cells expressing GFP (pooled from three biologic replicates).

ORGANISM(S): Mus musculus

SUBMITTER: Andy Chien 

PROVIDER: E-MTAB-3012 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress