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GPIF constrains Polycomb Repressive Complex 2 activity in germ cells

ABSTRACT: The project aimed at identifying the cofactors regulating Polycomb complex PRC2 enzymatic activity in gonads. We used a knock-in mouse model where the enzymatic subunit of PRC2 (either EZH2 or EZH1) is tagged (Flag-tag) to purify PRC2 from mouse adult testis. This experiment revealed the existence of a new cofactor for PRC2 that we called GPIF (AU022751).

INSTRUMENT(S): Orbitrap Fusion ETD, Q Exactive

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Testis, Cell Culture, Epithelial Cell Of Cervix, Bone Cell

DISEASE(S): Bone Osteosarcoma,Cervix Carcinoma

SUBMITTER: Pascal Jansen

LAB HEAD: Raphaël Margueron

PROVIDER: PXD012354 | Pride | 2019-07-22

REPOSITORIES: Pride

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EZHIP constrains Polycomb Repressive Complex 2 activity in germ cells.

Ragazzini Roberta R Pérez-Palacios Raquel R Baymaz Irem H IH Diop Seynabou S Ancelin Katia K Zielinski Dina D Michaud Audrey A Givelet Maëlle M Borsos Mate M Aflaki Setareh S Legoix Patricia P Jansen Pascal W T C PWTC Servant Nicolas N Torres-Padilla Maria-Elena ME Bourc'his Deborah D Fouchet Pierre P Vermeulen Michiel M Margueron Raphaël R

Nature communications 20190826 1

The Polycomb group of proteins is required for the proper orchestration of gene expression due to its role in maintaining transcriptional silencing. It is composed of several chromatin modifying complexes, including Polycomb Repressive Complex 2 (PRC2), which deposits H3K27me2/3. Here, we report the identification of a cofactor of PRC2, EZHIP (EZH1/2 Inhibitory Protein), expressed predominantly in the gonads. EZHIP limits the enzymatic activity of PRC2 and lessens the interaction between the cor ...[more]

PMID: 31451685

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