Dataset Information

Stylosanthes guianensis-LC-MS - Physiological responses and proteomic changes reveal insights into Stylosanthes response to manganese toxicity

ABSTRACT: Manganese (Mn), an essential element for plants, can be toxic when present in excess. Stylo (Stylosanthes) is a pioneer tropical legume with great potential for Mn tolerance, but its Mn tolerance mechanisms remain poorly understood. In this study, the mechanisms underlying stylo response to Mn toxicity were investigated using two stylo genotypes with contrasting Mn tolerance. Results showed that stylo genotype RY5 exhibited Mn tolerance superior to that of genotype TF2001, showing lower reductions in leaf chlorophyll concentration, maximum quantum yield of photosystem II (Fv/Fm) value and plant dry weight under Mn toxicity. Furthermore, RY5 tolerance to Mn toxicity could be attributed to stimulation of antioxidant protection and regulation of Mn homeostasis. Subsequently, a label-free quantitative proteomic analysis was conducted to investigate the protein profiles in the leaves and roots of stylo in response to Mn toxicity. A total of 356 differentially expressed proteins (DEPs) were identified, including 206 proteins from leaves and 150 proteins from roots, which consisted of 71 upregulated, 62 downregulated, 127 strongly induced and 96 completely suppressed proteins. These DEPs were mainly involved in defense response, photosynthesis, carbon fixation, metabolism, cell wall modulation and signaling. The qRT-PCR analysis verified that 10 out of 12 corresponding gene transcription patterns correlated with their encoding proteins after Mn exposure. Furthermore, stylo plants coped with Mn toxicity may be through enhancement of defense response and phenylpropanoid pathway, adjustment of metabolic process, and modulation of protein synthesis and turnover. Taken together, this study increases our understanding of tropical legume responses to Mn toxicity.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Stylosanthes Guianensis

TISSUE(S): Root, Leaf

SUBMITTER: Zhijian Chen

LAB HEAD: Zhijian Chen

PROVIDER: PXD012379 | Pride | 2019-05-06

REPOSITORIES: Pride

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Physiological responses and proteomic changes reveal insights into Stylosanthes response to manganese toxicity.

Liu Pandao P Huang Rui R Hu Xuan X Jia Yidan Y Li Jifu J Luo Jiajia J Liu Qin Q Luo Lijuan L Liu Guodao G Chen Zhijian Z

BMC plant biology 20190522 1

<h4>Background</h4>Manganese (Mn), an essential element for plants, can be toxic when present in excess. Stylo (Stylosanthes) is a pioneer tropical legume with great potential for Mn tolerance, but its Mn tolerance mechanisms remain poorly understood.<h4>Results</h4>In this study, variations in Mn tolerance were observed among nine stylo genotypes. Stylo genotype 'RY5' exhibited the highest Mn tolerance compared to the other tested genotypes, whereas 'TF2001' was a Mn-sensitive genotype. The mec ...[more]

PMID: 31113380

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Project description:BACKGROUND:Isobutanol is a promising next generation biofuel with demonstrated high yield microbial production, but the toxicity of this molecule reduces fermentation volumetric productivity and final titers. Organic solvent tolerance is a complex, multigenic phenotype that has been recalcitrant to rational engineering approaches. We apply experimental evolution followed by genome resequencing and a gene expression study to elucidate genetic bases on adaptation to exogenous isobutanol stress. RESULTS:The adaptations acquired in our evolved lineages exhibit antagonistic pleiotropy between minimal and rich medium, and appear to be specific to the effects of longer chain alcohols. By examining genotypic adaptation in multiple independent lineages, we find evidence of parallel evolution in hfq, mdh, acrAB, gatYZABCD, and rph genes. Many isobutanol tolerant lineages show reduced rpoS activity, perhaps related to mutations in hfq or acrAB. Consistent with the complex, multigenic nature of solvent tolerance, we observe adaptations in a diversity of cellular processes. Many adaptations appear to involve epistasis between different mutations, implying a rugged fitness landscape for isobutanol tolerance. We observe a trend of evolution targeting post-transcriptional regulation and high centrality nodes of biochemical networks. Collectively, the genotypic adaptations we observe suggest mechanisms of adaptation to isobutanol stress based on remodelling the cell envelope and surprisingly, stress response attenuation. CONCLUSIONS:We have discovered a set of genotypic adaptations that confer increased tolerance to exogenous isobutanol stress. Our results are immediately useful to efforts to engineer more isobutanol tolerant host strains of E. coli for isobutanol production. We suggest that rpoS and post-transcriptional regulators, such as hfq, RNA helicases, and sRNAs may be interesting mutagenesis targets for futurue global phenotype engineering. Two strains (WT strain and G3.2 mutant strain), each with two culture conditions (with and without isobutanol in medium). Three biological replicates for each strain/culture condition. Twelve samples in total.

Dataset Information

Stylosanthes guianensis-LC-MS - Physiological responses and proteomic changes reveal insights into Stylosanthes response to manganese toxicity

Publications

Physiological responses and proteomic changes reveal insights into Stylosanthes response to manganese toxicity.

Similar Datasets

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