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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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H3K27ac ChIPseq of the ecDNA-positive colon cancer cell line COLO 320DM with and without hydroxyurea treatment

ABSTRACT: Extrachromosomal DNA (ecDNA) is a major driver of oncogene amplification, intratumoural heterogeneity and rapid genetic change. We observe that ecDNA frequently undergoes segregation errors and is incorporated into micronuclei (MN) in ecDNA positive cancer cells. Different ecDNA species can coalesce into MN and lead to asymmetric inheritance. EcDNA in MN undergoes epigenetic changes and shows decreased oncogene transcription. Cells harbouring ecDNA-positive MN show prolonged cell cycle progression and an increased likelihood of cell death. Here we assess ecDNA H3K27ac occupancy after hydroxyurea treatment. COLO 320DM cells were treated with 80 µM hydroxyurea or DMSO (vehicle control) for 3 days and fixed for ChIPseq library preperation.

INSTRUMENT(S): Illumina NovaSeq X

ORGANISM(S): Homo sapiens

SUBMITTER: Robin Xu

PROVIDER: E-MTAB-16944 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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