Proteomics

Dataset Information

0

Human hippocampal spheroids for modelling AD

ABSTRACT: The hippocampus is important for memory formation and is severely affected in the brain with Alzheimer's disease (AD). Since AD brain tissue is available postmortem, our understanding of early pathogenic processes occurring in hippocampi remains speculative. Here, an MS-based proteomic approach was used to analyze free-floating hippocampal spheroids (HSs) enriched in PROX1-positive granule neurons, from induced pluripotent stem cells (iPSCs) of healthy individuals and AD patients. HSs generated from two AD patients carrying variations in amyloid precursor protein (APP) or presenilin 1 (PS1) genes, and their age and gender matched controls.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hippocampal Neuron, Cell Culture

DISEASE(S): Alzheimer's Disease

SUBMITTER: Melinda Rezeli  

LAB HEAD: György Marko-Varga

PROVIDER: PXD012524 | Pride | 2021-09-08

REPOSITORIES: Pride

Json Xml
altmetric image

Publications

The hippocampus is important for memory formation and is severely affected in the brain with Alzheimer disease (AD). Our understanding of early pathogenic processes occurring in hippocampi in AD is limited due to tissue unavailability. Here, we report a chemical approach to rapidly generate free-floating hippocampal spheroids (HSs), from human induced pluripotent stem cells. When used to model AD, both APP and atypical PS1 variant HSs displayed increased Aβ42/Aβ40 peptide ratios and decreased sy  ...[more]

Similar Datasets

Proteomics Patient-based model systems for AD
2023-10-24 | PXD042229 | Pride
Genomics 0fee821f-1280-4077-a8fa-5e90095beb00 - samples
| EGAD00001006886 | EGA
Unknown Whole hippocampus RNA seq on (SCDi Cayman 10566) and Vehicle (DMSO) in 10 month old WT and 3xTg-AD mice
2022-02-25 | GSE189693 | GEO
Proteomics Olfactory bulb proteome characterization in APP/PS1 mice
2016-08-09 | PXD003683 | Pride
Transcriptomics Expression data from Alzheimer's disease model mouse
2013-04-17 | E-GEOD-36981 | biostudies-arrayexpress
Unknown MTH1 and OGG1 maintain a low level of 8-oxoguanine in Alzheimer's brain, and prevent the progression of Alzheimer's pathogenesis
2021-03-24 | GSE83996 | GEO
Transcriptomics Expression data from post mortem Alzheimer's disease brains
2013-04-17 | E-GEOD-36980 | biostudies-arrayexpress
Unknown Gene expression analysis of astrocytes differentiated from iPS cells generated from the fibroblasts of PSEN1deltaE9 mutant AD and their isogenic control iPS lines
2019-12-31 | GSE138695 | GEO
Genomics Application of short-RNA-seq in post-mortem human hippocampi from the Calgary Brain Bank (CBB) to assess transcriptome-wide deregulation in processing of SINE Alu RNAs in Alzheimer’s disease
| EGAS00001004973 | EGA
Unknown Age-dependent changes in the gene expression profile of mice over-expressing mutant APP, PS1 and phosphorilated tau
2012-06-28 | GSE35210 | GEO