Proteomics

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Human lymphoma cell membrane proteomics by Pitchfork strategy

ABSTRACT: We combined glycopeptide enrichment by SPEG (Tian et al., 2007) with a hydrophobic segment-oriented hpTC method (Vít et al 2016) and a standard “detergent and trypsin” approach into a tree-pronged “Pitchfork” strategy for the analysis of membrane proteome in human lymphoma cells.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): B Cell, B-cell Lymphoma Cell

DISEASE(S): Lymphoma

SUBMITTER: Jiri Petrak  

LAB HEAD: Jiri Petrak

PROVIDER: PXD012647 | Pride | 2019-03-29

REPOSITORIES: Pride

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A three-pronged "Pitchfork" strategy enables an extensive description of the human membrane proteome and the identification of missing proteins.

Vit Ondrej O   Harant Karel K   Klener Pavel P   Man Petr P   Petrak Jiri J  

Journal of proteomics 20190606

Integral membrane proteins are under-represented in standard proteomic analyses, mostly because of their low expression and absence of trypsin-cleavage sites in their hydrophobic transmembrane segments. Novel and effective strategies for membrane proteomic analysis aim at soluble N-glycosylated segments of integral membrane proteins (CSC, SPEG, N-glyco-FASP) or selectively target the hydrophobic transmembrane alpha-helical segments employing chemical peptide cleavage by CNBr (hpTC). We combined  ...[more]

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