Proteomics

Dataset Information

0

Effect of MSC and MSC-derived extracellular vesicles on human blood coagulation


ABSTRACT: Mesenchymal stem cells (MSC) have emerged as potent therapeutic tool for a number of pathologies, including immune ones. However, unwelcome effects of MSC on the blood coagulation were revealed in some cases, which require more in-depth analysis. In this study, we explored the trombotic properties of human MSC from umbilical cord. We revealed strong procoagulant effects of umbilical cord MSC toward human and rat whole blood and platelets-free plasma using rotational thromboelastometry and thrombodynamics tests. The similar potentiation of clotting was demonstrated for MSC-derived extracellular vesicles (EV). In order to suggest approaches to avoid unwanted effects we studied the impact of heparin supplement on MSC/EV procoagulation properties. We found that therapeutic doses of unfractionated heparin injected in the patient's blood (administered in vivo) did not abrogate the procoagulant properties of MSC. Mass-spectrometry analysis of proteins of MSC and EV involved in coagulation-associated pathways was used to evaluate mechanisms of protrombotic effects.

INSTRUMENT(S): LTQ FT

ORGANISM(S): Homo sapiens  

TISSUE(S): Cell Culture

DISEASE(S): Not Available

SUBMITTER: Alexander Brzhozovskiy  

LAB HEAD: Alexey Kononikhin

PROVIDER: PXD012768 | Pride | 2019-03-26

REPOSITORIES: Pride

altmetric image

Publications


Mesenchymal stem cells (MSCs) have emerged as a potent therapeutic tool for the treatment of a number of pathologies, including immune pathologies. However, unwelcome effects of MSCs on blood coagulation have been reported, motivating us to explore the thrombotic properties of human MSCs from the umbilical cord. We revealed strong procoagulant effects of MSCs on human blood and platelet-free plasma using rotational thromboelastometry and thrombodynamic tests. A similar potentiation of clotting w  ...[more]

Similar Datasets

2020-04-09 | PXD015289 | Pride
| GSE99082 | GEO
2019-11-14 | PXD008926 | Pride
2015-05-07 | E-GEOD-59958 | ArrayExpress
| GSE83559 | GEO
2017-10-17 | PXD006048 | Pride
2018-05-17 | PXD007623 | Pride
2017-07-31 | PXD006197 | Pride
2018-05-21 | PXD006762 | Pride
2016-12-05 | PXD005351 | Pride