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Quantification of Tic-Toc subunits in gun1

ABSTRACT: Quantification of Tic-Toc subunits in the wild type and gun1-101 mutants in the control conditions and treated with lincomycin or norflurazon to determine if there are differences between genotypes.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Whole Body

SUBMITTER: Guo-Zhang Wu

LAB HEAD: Ralph Bock

PROVIDER: PXD013005 | Pride | 2019-04-23

REPOSITORIES: Pride

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Publications

Control of retrograde signalling by protein import and cytosolic folding stress.

Wu Guo-Zhang GZ Meyer Etienne H EH Richter Andreas S AS Schuster Maja M Ling Qihua Q Schöttler Mark A MA Walther Dirk D Zoschke Reimo R Grimm Bernhard B Jarvis R Paul RP Bock Ralph R

Nature plants 20190506 5

Communication between organelles and the nucleus is essential for fitness and survival. Retrograde signals are cues emitted from the organelles to regulate nuclear gene expression. GENOMES UNCOUPLED1 (GUN1), a protein of unknown function, has emerged as a central integrator, participating in multiple retrograde signalling pathways that collectively regulate the nuclear transcriptome. Here, we show that GUN1 regulates chloroplast protein import through interaction with the import-related chaperon ...[more]

PMID: 31061535

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Expression data from highly purified MMTV-Neu Tumor Initiating Cells (TICs) and the non-TIC CD24- fraction

Project description:The cancer stem cell model maintains that tumors are organized in a hierarchy driven by tumor initiating cells (TICs), and that patient survival inversely correlates with TIC gene expression. Here we generated a prognostic signature for HER2+ breast cancer from TICs purified from MMTV-Her2/Neu mammary tumors. TICs from this model, identified as Lin-:CD24+:JAG1- at a frequency of 2-5% by serial and single cell transplantation assays, showed elevated expression of proliferation genes and low expression of differentiation genes (compared to non-TIC fraction CD24- of the same tumor). We used microarrays to detect differentially expressed genes in the TIC fraction compared to the non-TIC fraction of the same tumor Cells from each primary tumor were separated by FACS into TIC and non-TIC fractions and total RNA was extracted and hybridized on Affymetrix microarrays.

2011-05-28 | E-GEOD-29590 | biostudies-arrayexpress

lincomycin treatment wild type and gun1-101

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2019-06-05 | PXD011759 | Pride

Dawn anticipation

Project description:To investigate the role of TIC in the morning perception process, we performed a transcriptome analysis of the wild type and time for coffee mutant (tic-2) at dawn. An additional file is included. In this file, the expression values for the replicates are condensed in a single data point obtaining one mean and standard deviation of the expression values per genotype. The protocol for this file is: log2 fold change followed by a False Discovery Rate with a p-value equal or below 0.05.

2013-10-10 | E-MTAB-1362 | biostudies-arrayexpress

The chloroplast outer and inner envelope membrane translocase (TOC/TIC) interaction network identifies a 23 kDa glycine-rich protein as new subunit of the 1-MDa TIC complex

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2019-02-22 | PXD008469 | Pride

ZFHX4 and CHD4 ChIP sequencing in glioblastoma tumor initiating cells

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2014-01-16 | E-GEOD-52419 | biostudies-arrayexpress

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2023-03-11 | PXD035701 | Pride

Transcription profiling of Arabidopsis col0,gun1,abi4-102, treated with and without lincomycin

Project description:To test the relationship between GUN1 and ABI4 Experiment Overall Design: 2*3 factorial design, two factors (1) treatment: with/without lincomycin (2) genotype: col0, gun1, abi4-102

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Project description: Not available

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Project description: Not available

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Long non-coding RNA produced by RNA polymerase V determines boundaries of heterochromatin

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