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Eukaryotic Ribosome GCN2 interaction Hydrogen/Deuterium Exchange Mass Spectrometry

ABSTRACT: General control nonderepressible 2 (GCN2) phosphorylates eIF2α, regulating translation in response to nutritional stress. Here, we show that mammalian ribosomes are potent GCN2 activators. Hydrogen/deuterium exchange–mass spectrometry (HDX-MS) showed GCN2 interacting with domain II of the uL10 P-stalk protein. The P-stalk is a uL10/P12/P22 pentameric complex that is part of the ribosomal GTPase-associated center. Recombinant human P-stalk greatly stimulates GCN2. Both domain II of uL10 and the C-terminal tails of P1 and P2 are necessary for maximal GCN2 activation. On actively translating ribosomes, the C-terminal tails of P1 and P2 are sequestered by elongation factors, suggesting P-stalk availability could link translational stress to GCN2 activation.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Oryctolagus Cuniculus (rabbit)

TISSUE(S): Blood Cell, Blood

SUBMITTER: Glenn Masson

LAB HEAD: Roger L. Williams

PROVIDER: PXD013051 | Pride | 2019-05-24

REPOSITORIES: Pride

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Activation of GCN2 by the ribosomal P-stalk.

Inglis Alison J AJ Masson Glenn R GR Shao Sichen S Perisic Olga O McLaughlin Stephen H SH Hegde Ramanujan S RS Williams Roger L RL

Proceedings of the National Academy of Sciences of the United States of America 20190225 11

Cells dynamically adjust their protein translation profile to maintain homeostasis in changing environments. During nutrient stress, the kinase general control nonderepressible 2 (GCN2) phosphorylates translation initiation factor eIF2α, initiating the integrated stress response (ISR). To examine the mechanism of GCN2 activation, we have reconstituted this process in vitro, using purified components. We find that recombinant human GCN2 is potently stimulated by ribosomes and, to a lesser extent, ...[more]

PMID: 30804176

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