Proteomics

Dataset Information

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786-O ubiquitiome - Loss of BAP1 Results in Growth Inhibition and Enhances Mesenchymal-Epithelial Transition in Kidney Tumor Cells

ABSTRACT: 786-O WT and BAP1-KO cells were cultured in heavy (13C6-L-arginine, 13C6-L-lysine, Cambridge Isotope Lab) and light (unlabeled L-arginine and L-lysine) SILAC RPMI-1640 medium (Thermo Fisher Scienific), respectively. After more than 10 passages, cells were lysed with 8 M urea and 5 mg protein from each group were mix. The ubiquitinated peptide enrichment was performed using PTMScan® (Cell Signaling Technology, #5562) following the manufacturer’s instructions.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Kidney

DISEASE(S): Renal Clear Cell Carcinoma

SUBMITTER: Pengsheng Chen  

LAB HEAD: Haiteng Deng

PROVIDER: PXD013127 | Pride | 2019-04-18

REPOSITORIES: Pride

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Publications

Loss of BAP1 Results in Growth Inhibition and Enhances Mesenchymal-Epithelial Transition in Kidney Tumor Cells.

Chen Pengsheng P   Wang Huan H   Zhang Wenhao W   Chen Yuling Y   Lv Yang Y   Wu Di D   Guo Mingzhou M   Deng Haiteng H  

Molecular & cellular proteomics : MCP 20190416 7

BRCA1-associated protein 1 (BAP1) is a member of the ubiquitin C-terminal hydrolase family of deubiquitinating enzymes and is implicated in transcriptional regulation. The <i>BAP1</i> gene is mutated in about 10% of patients with ccRCC, the most common form of renal cancer, suggesting that BAP1 is a tumor suppressor. However, whether <i>BAP1</i> influences the progression of ccRCC tumors expressing wild-type (WT) <i>BAP1</i> is unclear. Here, we assessed the expression and function of BAP1 using  ...[more]