Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Schizosaccharomyces Pombe 927
SUBMITTER: Danny Bergeron
LAB HEAD: Francois Bachand
PROVIDER: PXD013183 | Pride | 2019-05-16
REPOSITORIES: Pride
Larochelle Marc M Bergeron Danny D Arcand Bruno B Bachand François F
Journal of cell science 20190531 11
The use of proximity-dependent biotinylation assays coupled to mass spectrometry (PDB-MS) has changed the field of protein-protein interaction studies. However, despite the recurrent and successful use of BioID-based protein-protein interactions screening in mammalian cells, the implementation of PDB-MS in yeast has not been effective. Here, we report a simple and rapid approach in yeast to effectively screen for proximal and interacting proteins in their natural cellular environment by using Tu ...[more]