Proteomics

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Daedalus and Gasz recruit Armitage to mitochondria, bringing piRNA precursors to the biogenesis machinery


ABSTRACT: The piRNA pathway is a small RNA-based immune system that silences mobile genetic elements in animal germlines. piRNA biogenesis requires a specialised machinery that converts long single-stranded precursors into small RNAs of ~25-nucleotides in length. This process involves factors that operate in two different subcellular compartments: the nuage/Yb-bodies and mitochondria. How these two sites communicate to achieve accurate substrate selection and efficient processing remains unclear. Here, we investigate a previously uncharacterized piRNA biogenesis factor, Daedalus (Daed), that is located on the outer mitochondrial membrane. Daed is essential for Zucchini-mediated piRNA production and for the correct localisation of the indispensable piRNA biogenesis factor, Armitage (Armi). We find that Gasz and Daed interact with each other and likely provide a mitochondrial “anchoring platform” to ensure that Armi is held in place, proximal to Zucchini, during piRNA processing. Our data suggest that Armi initially identifies piRNA precursors in nuage/Yb-bodies in a manner that depends upon Piwi and then moves to mitochondria to present precursors to the mitochondrial biogenesis machinery. These results represent a significant step in understanding a critical aspect of transposon silencing, namely how RNAs are chosen to instruct the piRNA machinery in the nature of its silencing targets.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Drosophila melanogaster  

TISSUE(S): Cell Culture

DISEASE(S): Not Available

SUBMITTER: Marzia Munafo  

LAB HEAD: Gregory J Hannon

PROVIDER: PXD013403 | Pride | 2019-04-18

REPOSITORIES: Pride

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Publications

Daedalus and Gasz recruit Armitage to mitochondria, bringing piRNA precursors to the biogenesis machinery.

Munafò Marzia M   Manelli Vera V   Falconio Federica A FA   Sawle Ashley A   Kneuss Emma E   Eastwood Evelyn L EL   Seah Jun Wen Eugene JWE   Czech Benjamin B   Hannon Gregory J GJ  

Genes & development 20190523 13-14


The Piwi-interacting RNA (piRNA) pathway is a small RNA-based immune system that silences mobile genetic elements in animal germlines. piRNA biogenesis requires a specialized machinery that converts long single-stranded precursors into small RNAs of ∼25-nucleotides in length. This process involves factors that operate in two different subcellular compartments: the nuage/Yb body and mitochondria. How these two sites communicate to achieve accurate substrate selection and efficient processing rema  ...[more]

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