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Inhibition of mPGES-1 or COX-2 Results in Different Proteomic and Lipidomic Profiles in A549 Lung Cancer Cells

ABSTRACT: We compared the effects of mPGES-1 inhibitor Compound III (CIII) with the cyclooxygenase (COX)-2 inhibitor NS-398 on protein and lipid profiles in interleukin (IL)-1β induced A549 lung cancer cells using mass spectrometry. Inhibition of mPGES-1 decreased PGE2 production and increased PGF2α and thromboxane B2 (TXB2) formation, while inhibition of COX-2 decreased the production of all three prostanoids. Our proteomics results revealed that CIII downregulated multiple canonical pathways including eIF2, eIF4/P70S6K, and mTOR signaling, compared to NS-398 that activated these pathways.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell Of Lung, Cell Culture

DISEASE(S): Lung Cancer

SUBMITTER: Elena Ossipova

LAB HEAD: Per-Johan Jakobsson

PROVIDER: PXD013490 | Pride | 2019-05-20

REPOSITORIES: Pride

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Inhibition of mPGES-1 or COX-2 Results in Different Proteomic and Lipidomic Profiles in A549 Lung Cancer Cells.

Bergqvist Filip F Ossipova Elena E Idborg Helena H Raouf Joan J Checa Antonio A Englund Karin K Englund Petter P Khoonsari Payam Emami PE Kultima Kim K Wheelock Craig E CE Larsson Karin K Korotkova Marina M Jakobsson Per-Johan PJ

Frontiers in pharmacology 20190607

Pharmacological inhibition of microsomal prostaglandin E synthase (mPGES)-1 for selective reduction in prostaglandin E<sub>2</sub> (PGE<sub>2</sub>) biosynthesis is protective in experimental models of cancer and inflammation. Targeting mPGES-1 is envisioned as a safer alternative to traditional non-steroidal anti-inflammatory drugs (NSAIDs). Herein, we compared the effects of mPGES-1 inhibitor Compound III (CIII) with the cyclooxygenase (COX)-2 inhibitor NS-398 on protein and lipid profiles in ...[more]

PMID: 31231223

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