Dataset Information

System OMICs analysis of Mycobacterium tuberculosis Beijing B0/W148 cluster

ABSTRACT: Mycobacterium tuberculosis Beijing B0/W148 is one of the most widely distributed clusters in the Russian Federation and in some countries of the former Soviet Union. Recent studies have improved our understanding of the reasons for the “success” of the cluster but this area remains incompletely studied. Here, we focused on the system omics analysis of the RUS_B0 strain belonging to the Beijing B0/W148 cluster. Completed genome sequence of RUS_B0 (CP020093.1) and a collection of WGS for 200 cluster strains from the NCBI were used to describe the main genetic features of the population, as well as the level of resistance. In turn, proteome and transcriptome studies allowed to confirm the genomic data and to identify a number of finds that have not previously been described. Our results demonstrate that expression of the whiB6 which contains cluster-specific polymorphism (T4338371G) increased by more than 50 times in RUS_B0. Additionally, the level of ethA transcripts in RUS_B0 is increased almost 30 times compared to the H37Rv. Start sites for 10 genes were corrected based on the combination of proteomic and transcriptomic data. Additionally, based on the omics approach, we identified 5 new genes.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mycobacterium Tuberculosis

SUBMITTER: Alexander Smolyakov

LAB HEAD: Shitikov E.A.

PROVIDER: PXD013509 | Pride | 2020-05-26

REPOSITORIES: Pride

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System OMICs analysis of Mycobacterium tuberculosis Beijing B0/W148 cluster.

Bespyatykh Julia J Shitikov Egor E Guliaev Andrei A Smolyakov Alexander A Klimina Ksenia K Veselovsky Vladimir V Malakhova Maya M Arapidi Georgij G Dogonadze Marine M Manicheva Olga O Bespiatykh Dmitry D Mokrousov Igor I Zhuravlev Viacheslav V Ilina Elena E Govorun Vadim V

Scientific reports 20191217 1

Mycobacterium tuberculosis Beijing B0/W148 is one of the most widely distributed clusters in the Russian Federation and in some countries of the former Soviet Union. Recent studies have improved our understanding of the reasons for the "success" of the cluster but this area remains incompletely studied. Here, we focused on the system omics analysis of the RUS_B0 strain belonging to the Beijing B0/W148 cluster. Completed genome sequence of RUS_B0 (CP020093.1) and a collection of WGS for 394 clust ...[more]

PMID: 31848428

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Dataset Information

System OMICs analysis of Mycobacterium tuberculosis Beijing B0/W148 cluster

Publications

System OMICs analysis of Mycobacterium tuberculosis Beijing B0/W148 cluster.

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