Proteomics

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Immunoglobulin V-gene usage among human serum IgA with or without specificity to the autoantigen transglutaminase 2


ABSTRACT: To study V-gene usage among antigen-specific and non-antigen-specific serum IgA antibodies in celiac disease patients, purified antibody fractions were subjected to proteomic analysis. Patients either had eastablished, untreated disease with severe intestinal inflammation or "potential" celiac disease characterized by presence of antibodies but no or mild inflammation. Quantification of the fraction of antibodies using different IGHV segments revealed that antibodies specific to the autoantigen transglutaminase 2 (TG2) displayed a bias toward usage of IGHV5-51 in agreement with previous observations. The fraction of antibodies using IGHV5-51 correlated with the degree of intestinal inflammation, indicating that production of hallmark IGHV5-51 anti-TG2 antibodies coincides with onset of clinical celiac disease.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo sapiens  

TISSUE(S): Blood Serum

DISEASE(S): Celiac Disease

SUBMITTER: Rasmus Iversen  

LAB HEAD: Ludvig M. Sollid

PROVIDER: PXD013777 | Pride | 2019-07-09

REPOSITORIES: Pride

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