Dataset Information

Antagonistic interactions between benzo[a]pyrene and C60 in toxicological response of Marine Mussel

ABSTRACT: his integrated study aimed to assess the ecotoxicological effects of the interaction of fullerene (C60) and benzo[a]pyrene (B[a]P) on the marine mussel, Mytilus galloprovincialis. The uptake of nC60, B[a]P and mixtures of nC60 and B[a]P into mussel tissues was confirmed by GC-MS, LC-HRMS and ICP-MS. Biomarkers of DNA damage as well as proteomics analysis were applied to unravel the toxic effect of B[a]P and C60. Antagonistic responses were observed at the genotoxic and proteomic level. Differentially expressed proteins (DEPs) were only identified in the B[a]P single exposure and the B[a]P mixture exposure groups containing 1 mg/L of C60, the majority of which were down-regulated (~52 %). No DEPs were identified at any of the concentrations of nC60 (p < 0.05, 1 % FDR). Using DEPs identified at a threshold of (p < 0.05; B[a]P and B[a]P mixture with nC60), gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis indicated that these proteins were enriched with a broad spectrum of biological processes and pathways, including those broadly associated with protein processing, cellular processes and environmental information processing. Among those significantly enriched pathways, the ribosome was consistently the top enriched term irrespective of treatment or concentration and plays an important role as the site of biological protein synthesis and translation. Our results demonstrate the complex multi-modal response to environmental stressors in M. galloprovincialis.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mytilus Galloprovincialis (mediterranean Mussel)

SUBMITTER: Laura Langan

LAB HEAD: Awadhesh N. Jha

PROVIDER: PXD013805 | Pride | 2019-07-18

REPOSITORIES: Pride

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Publications

Antagonistic Interactions between Benzo[a]pyrene and Fullerene (C60) in Toxicological Response of Marine Mussels.

Barranger Audrey A Langan Laura M LM Sharma Vikram V Rance Graham A GA Aminot Yann Y Weston Nicola J NJ Akcha Farida F Moore Michael N MN Arlt Volker M VM Khlobystov Andrei N AN Readman James W JW Jha Awadhesh N AN

Nanomaterials (Basel, Switzerland) 20190708 7

This study aimed to assess the ecotoxicological effects of the interaction of fullerene (C60) and benzo[a]pyrene (B[a]P) on the marine mussel, Mytilus galloprovincialis. The uptake of nC60, B[a]P and mixtures of nC60 and B[a]P into tissues was confirmed by Gas Chromatography-Mass Spectrometry (GC-MS), Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS) and Inductively Coupled Plasma Mass Spectrometer (ICP-MS). Biomarkers of DNA ...[more]

PMID: 31288459

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Dataset Information

Antagonistic interactions between benzo[a]pyrene and C60 in toxicological response of Marine Mussel

Publications

Antagonistic Interactions between Benzo[a]pyrene and Fullerene (C<sub>60</sub>) in Toxicological Response of Marine Mussels.

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