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Role of GATA-2 -77 enhancer in murine blood progenitor cells

ABSTRACT: We discovered enhancers that control GATA-2 expression during mouse development and in adult and generated mutant mouse strains that lack these enhancers, and these mice have very specific and important phenotypes that revealed GATA-2 mechanisms to control stem and progenitor cell transitions. In this study we aim to determine what proteins are under control of the GATA-2 -77 enhancer in a pool of heterogeneous blood progenitor cells with the specific focus on Socs2 expression.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Blood Cell, Primary Cell

SUBMITTER: Evgenia Shishkova

LAB HEAD: Joshua Coon

PROVIDER: PXD013855 | Pride | 2020-06-05

REPOSITORIES: Pride

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Constructing and deconstructing GATA2-regulated cell fate programs to establish developmental trajectories.

Johnson Kirby D KD Conn Daniel J DJ Shishkova Evgenia E Katsumura Koichi R KR Liu Peng P Shen Siqi S Ranheim Erik A EA Kraus Sean G SG Wang Weixin W Calvo Katherine R KR Hsu Amy P AP Holland Steven M SM Coon Joshua J JJ Keles Sunduz S Bresnick Emery H EH

The Journal of experimental medicine 20201101 11

Stem and progenitor cell fate transitions constitute key decision points in organismal development that enable access to a developmental path or actively preclude others. Using the hematopoietic system, we analyzed the relative importance of cell fate-promoting mechanisms versus negating fate-suppressing mechanisms to engineer progenitor cells with multilineage differentiation potential. Deletion of the murine Gata2-77 enhancer, with a human equivalent that causes leukemia, downregulates the tra ...[more]

PMID: 32736380

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