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Comparative integrated omics analysis of the Hfq regulon in Bordetella pertussis

ABSTRACT: This study represents the first attempt to characterize the RNA chaperone Hfq regulon in bacterial pathogen using an integrative omics approach. Gene expression profiles were analyzed by RNA-seq and protein amounts in cell-associated and cell-free fractions were determined by LC-MS/MS technique. Comparative analysis of transcriptomic and proteomic data revealed solid correlation considering the role of Hfq in post-transcriptional control of gene expression. Importantly, our study confirms and further enlightens the role of Hfq in pathogenicity of B. pertussis.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Bordetella Pertussis (strain Tohama I / Atcc Baa-589 / Nctc 13251)

SUBMITTER: Denisa Petrackova

LAB HEAD: Branislav Vecerek

PROVIDER: PXD013953 | Pride | 2019-07-02

REPOSITORIES: Pride

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Comparative Integrated Omics Analysis of the Hfq Regulon in Bordetella pertussis.

Dienstbier Ana A Amman Fabian F Štipl Daniel D Petráčková Denisa D Večerek Branislav B

International journal of molecular sciences 20190624 12

Bordetella pertussis is a Gram-negative strictly human pathogen of the respiratory tract and the etiological agent of whooping cough (pertussis). Previously, we have shown that RNA chaperone Hfq is required for virulence of B. pertussis. Furthermore, microarray analysis revealed that a large number of genes are affected by the lack of Hfq. This study represents the first attempt to characterize the Hfq regulon in bacterial pathogen using an integrative omics approach. Gene expressi ...[more]

PMID: 31238496

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