Proteomics

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The Response of A549 Cells to Aspergillus fumigatus and Pseudomonas aeruginosa: A Proteomic perspective


ABSTRACT: Aspergillus fumigatus and Pseudomonas aeruginosa are the most prevalent fungal and bacterial pathogens, associated with cystic fibrosis-related infections, respectively. Although it is well established that P. aeruginosa eventually predominates as the primary pathogen, it is unknown why this is the case. Label-free quantitative proteomics was employed here to compare the cellular response to A. fumigatus, P. aeruginosa and sequential exposure to both pathogens in the type II alveolar epithelial cell line, A549, a model of the alveolar surface. In total, 2264 proteins were identified and the analysis of statistically significant (p<0.05; ±1.5 fold change), differentially abundant (SSDA) proteins, revealed an increase in the relative abundance of proteins associated with biological processes common to all pathogen-exposed groups. These included mitochondrial stress, energy metabolism and protein processing in the endoplasmic reticulum. Conversely, clear differences were observed between the cellular response to A. fumigatus or P. aeruginosa and following sequential exposure to both pathogens. The relative abundance of proteins associated with ribosomal activity appeared comparable between sequentially exposed cells and unexposed cells, but were substantially decreased in fungal- and bacterial-exposed cells. Furthermore, compared to unexposed cells, the relative abundance of proteins associated with the actin cytoskeleton, endocytosis, phagosome and lysosome were increased in A. fumigatus and P. aeruginosa-exposed cells, but appeared unchanged or decreased in sequentially exposed cells. This research provides novel insights into the whole cell proteomic response to A. fumigatus and P. aeruginosa, and highlights distinct differences following sequential exposure to both pathogens.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: James Carolan  

LAB HEAD: James Carolan

PROVIDER: PXD014022 | Pride | 2019-11-11

REPOSITORIES: Pride

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Publications

Characterization of the Proteomic Response of A549 Cells Following Sequential Exposure to <i>Aspergillus fumigatus</i> and <i>Pseudomonas aeruginosa</i>.

Margalit Anatte A   Kavanagh Kevin K   Carolan James C JC  

Journal of proteome research 20191114 1


<i>Aspergillus fumigatus</i> and <i>Pseudomonas aeruginosa</i> are the most prevalent fungal and bacterial pathogens associated with cystic-fibrosis-related infections, respectively. <i>P. aeruginosa</i> eventually predominates as the primary pathogen, though it is unknown why this is the case. Label-free quantitative proteomics was employed to investigate the cellular response of the alveolar epithelial cell line, A549, to coexposure of <i>A. fumigatus</i> and <i>P. aeruginosa</i>. These studie  ...[more]

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