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Bacillus amyloliquefaciens Ba168 LC-MSMS

ABSTRACT: To establish the protein expression profile of Ba168, a high-resolution LC-MS/MS proteomic analysis was performed. A total of 1155 proteins were identified from 5233 unique peptides. GO and KEGG analysis revealed that a majority of the proteins were associated with biosynthesis and carbon metabolism pathways, such as biosynthesis of amino acids, peptidoglycan, and antibiotics. Then, we identified the antimicrobial proteins of Ba168. At least 16 potential antimicrobial-activity-related proteins were identified; 11 of these proteins have direct antimicrobial effects, while 5 of these proteins are associated with the formation of antimicrobial substances.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Bacillus Amyloliquefaciens

TISSUE(S): Cell Culture

SUBMITTER: meihuan lu

LAB HEAD: Meihuan Lu

PROVIDER: PXD014057 | Pride | 2022-08-11

REPOSITORIES: Pride

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Publications

Analysis and Evaluation of the Flagellin Activity of Bacillus amyloliquefaciens Ba168 Antimicrobial Proteins against Penicillium expansum.

Lu Meihuan M Chen Yahan Y Li Lijun L Ma Yinghui Y Tong Zefang Z Guo Dongsheng D Sun Pingping P An Derong D

Molecules (Basel, Switzerland) 20220701 13

Blue mold caused by Penicillium expansum is one of the most common apple diseases, and it is becoming a serious threat in apple production. The strain Bacillus amyloliquefaciens Ba168 showed high levels of antimicrobial activity in our previous study. To analyze the antimicrobial protein of Ba168, a high-resolution LC-MS/MS proteomic analysis was performed. A total of 1155 proteins were identified from 5233 unique peptides. A total of 16 potential antimicrobial-activity-related proteins w ...[more]

PMID: 35807503

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