Time-resolved phosphoproteomic analysis of the TCR signaling pathway in primary T cells
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Spleen, Primary Cell, T Cell, Lymph Node
SUBMITTER: 
Anne Gonzalez de Peredo
LAB HEAD: Odile Schiltz
PROVIDER: PXD014225 | Pride | 2020-07-09
REPOSITORIES: Pride
Publications
LymphoAtlas: a dynamic and integrated phosphoproteomic resource of TCR signaling in primary T cells reveals ITSN2 as a regulator of effector functions.
Locard-Paulet Marie M Voisinne Guillaume G Froment Carine C Goncalves Menoita Marisa M Ounoughene Youcef Y Girard Laura L Gregoire Claude C Mori Daiki D Martinez Manuel M Luche Hervé H Garin Jerôme J Malissen Marie M Burlet-Schiltz Odile O Malissen Bernard B Gonzalez de Peredo Anne A Roncagalli Romain R
Molecular systems biology 20200701 7
T-cell receptor (TCR) ligation-mediated protein phosphorylation regulates the activation, cellular responses, and fates of T cells. Here, we used time-resolved high-resolution phosphoproteomics to identify, quantify, and characterize the phosphorylation dynamics of thousands of phosphorylation sites in primary T cells during the first 10 min after TCR stimulation. Bioinformatic analysis of the data revealed a coherent orchestration of biological processes underlying T-cell activation. In particu ...[more]
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