Proteomics

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Molecular basis of b-adrenergic regulation of cardiac calcium current


ABSTRACT: Increased cardiac contractility during fight-or-flight response is caused by b-adrenergic augmentation of CaV1.2 channels. It is assumed that this iconic regulation involves phosphorylation of CaV1.2 a1/b-subunits. In transgenic murine hearts expressing fully PKA phosphorylation-deficient mutant a1C/b, this regulation persists, however, suggesting involvement of extra-channel factors. We here show that PKA up-regulates cardiac CaV1.2 channels by phosphorylating the small G-protein Rad, relieving constitutive inhibition of CaV1.2. Peroxidase-catalyzed labeling in mice hearts expressing ascorbate peroxidase conjugated-a1C or b2b with multiplexed quantitative proteomics allowed tracking of thousands of proteins in proximity of CaV1.2.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Larimichthys crocea   Xanthomonas oryzae pv. oryzae PXO99A   Peniophora incarnata   Lysiphlebus fabarum   Lentinus crinitus   Rhodopseudomonas palustris   Coxiella burnetii RSA 493   Stenotrophomonas maltophilia   Aspergillus nidulans TN02A3   Nelumbo nucifera   Bothrops neuwiedi   Actinomycetia   Medicago truncatula   Pyrococcus abyssi   Staphylococcus sp. 1   Tetrahymena thermophila   Bacillus subtilis subsp. subtilis str. 168   Oreochromis mossambicus   Flavobacterium columnare   Burkholderia cenocepacia   Rhodococcus jostii RHA1   Aves   Desulfovibrio ferrophilus   Giardia intestinalis   Struthio camelus   Schistosoma mansoni   Muvirus mu   Trypanosoma   Streptococcus pneumoniae TIGR4   Sus scrofa   Desulfovibrio sp. 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Leaf257   Human alphaherpesvirus 1 strain 17   Zymomonas mobilis   Anopheles culicifacies A   Tuber albidum   Pseudomonas pseudoalcaligenes CECT 5344   Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10   Mustela putorius furo   Oncorhynchus tshawytscha   Crithidia fasciculata   Arachis hypogaea   Riptortus pedestris   Plasmodium sp. pBT9   Coffea eugenioides   Olavius algarvensis Gamma 3 endosymbiont   Physcomitrella   Lactococcus lactis subsp. cremoris MG1363   Cyclopes didactylus   Human alphaherpesvirus 1 strain KOS   soil bacterium TS1114R   Zea mays   Brugia malayi   Lactococcus lactis subsp. cremoris UC509.9   Coffea canephora   Bothrops jararacussu   Phytophthora capsici LT1534   Anopheles culicifacies B   Bartonella quintana JK 31   Thalassiosira pseudonana CCMP1335   Ficus carica   Human immunodeficiency virus 1   Globicatella sanguinis   Crassostrea hongkongensis   Tapirus terrestris   Olavius algarvensis Delta 1 endosymbiont   Severe acute respiratory syndrome coronavirus 2   Cytomegalovirus   Rhizobium cf. leguminosarum   Haptophyta   Thermochaetoides thermophila   Rattus norvegicus   Homo sapiens subsp. 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ELBA   Streptococcus dysgalactiae subsp. equisimilis SK1249   Vitis   Acinetobacter baumannii ACICU   Human herpesvirus 2 strain 186   Oryctolagus cuniculus cuniculus   [Candida] glabrata   Lactococcus phage   Toxoplasma gondii type III   Physcomitrium patens   Burkholderia cenocepacia K56-2Valvano   Clostridium autoethanogenum   Hebomoia glaucippe   Nephila clavipes   Alteromonadales   Streptococcus pneumoniae SP14-BS69   Xenopus laevis   Mycobacterium tuberculosis SP13   anaerobic digester metagenome   Naja mandalayensis   Tarenaya hassleriana   Brachyspiraceae   Paracoccidioides brasiliensis Pb18   Streptococcus pneumoniae D39   hybrid   Macaca fascicularis   Microcystis aeruginosa   Phytophthora pisi   Oncorhynchus mykiss   Acanthamoeba castellanii   Burkholderia sp. 2332   Ursus arctos arctos   Leishmania infantum JPCM5   Chlorocebus aethiops   Equus asinus   Morchella importuna   Helianthus annuus   Aerococcus urinae NBRC 15544 = CCUG 36881   Brucella abortus   Mycoplasma pneumoniae M129-B7   Mycoplasmopsis agalactiae   Tuber indicum   Austrolebias   Chlamydia trachomatis 434/Bu   Fasciola hepatica   Apis mellifera   Bos taurus   Viruses   Drosophila melanogaster   Catenibacterium   Pyricularia oryzae B157   Teleogryllus oceanicus   Acanthoscurria gomesiana   Camelus dromedarius   Populus yunnanensis   Pseudomonas aeruginosa   Ustilago maydis   Cricetulus griseus   Mytilus edulis   Sphingobium chlorophenolicum   Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044   Passiflora edulis   Heloderma suspectum suspectum   Cryptococcus neoformans var. grubii H99   Caenorhabditis elegans   Daktulosphaira vitifoliae   Mengo virus   Oryza sativa   Beta vulgaris subsp. vulgaris   mixed culture metagenome   Drosophila   Rhizobium sp. Leaf68   Populus trichocarpa   Strawberry leafy fruit phytoplasma   Vibrio cholerae O1 biovar El Tor str. N16961   Cupriavidus metallidurans   Trichoderma reesei RUT C-30   Scophthalmus maximus   Brassica oleracea var. italica   Octopodiformes   Listeria monocytogenes EGD-e   Ophrys x arachnitiformis subsp. archipelagi   Tuber aestivum   Mycobacterium tuberculosis   Brucella   Gallus gallus   Salmo sp.   Homo sapiens   Trichomonas gallinae   Aspergillus flavus   Malus domestica   Helicobacter pylori 26695   Mylodon darwinii   Toxoplasma gondii   Butyrivibrio fibrisolvens   Mycobacterium marinum ATCC BAA-535   Lithobius forficatus   Mycobacterium avium subsp. paratuberculosis K-10   Canis lupus familiaris   Dioscorea oppositifolia   Staphylococcus haemolyticus   Danio rerio   Ascaris suum   Acinetobacter baumannii AB307-0294   Quercus ilex  

SUBMITTER: Marian Kalocsay  

LAB HEAD: Marian Kalocsay

PROVIDER: PXD014500 | Pride | 2019-11-19

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
336300.mzXML Mzxml
336301.mzXML Mzxml
336302.mzXML Mzxml
336303.mzXML Mzxml
336304.mzXML Mzxml
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Publications


Increased cardiac contractility during the fight-or-flight response is caused by β-adrenergic augmentation of Ca<sub>V</sub>1.2 voltage-gated calcium channels<sup>1-4</sup>. However, this augmentation persists in transgenic murine hearts expressing mutant Ca<sub>V</sub>1.2 α<sub>1C</sub> and β subunits that can no longer be phosphorylated by protein kinase A-an essential downstream mediator of β-adrenergic signalling-suggesting that non-channel factors are also required. Here we identify the mec  ...[more]

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