Dataset Information


Structural complementarity offsets low receptor affinity in E7820-mediated RBM39 degradation by CRL4DCAF15

ABSTRACT: The investigational drugs E7820, indisulam and tasisulam (aryl-sulfonamides) promote the degradation of the splicing factor RBM39 in a proteasome and CRL4DCAF15 ubiquitin ligase-dependent mechanism, however the molecular details of this activity remain elusive. Here we present the cryo-EM structure of DDB1-DCAF15-DDA1 bound to RBM39 and E7820 at 4.4 Å resolution, together with crystal structures of engineered subcomplexes. We show that DCAF15 adopts a novel fold stabilized by DDA1, and that extensive protein-protein contacts between the ligase and substrate mitigate the low affinity interaction between aryl-sulfonamides and DCAF15. Our data demonstrates how aryl-sulfonamides neo-functionalize a shallow, non-conserved pocket on DCAF15 to selectively bind and degrade RBM39 and RBM23 without the requirement for a high affinity ligand, which has broad implications for the de novo discovery of molecular glue degraders.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo sapiens  

TISSUE(S): Cell Culture

DISEASE(S): Neuroblastoma

SUBMITTER: Eric Fischer  

PROVIDER: PXD014536 | Pride | 2019-07-10


Similar Datasets

2018-08-10 | PXD010428 | Pride
2018-08-09 | PXD010417 | Pride
2018-08-09 | PXD010416 | Pride
2018-08-09 | PXD010418 | Pride
2018-08-09 | PXD010420 | Pride
2019-01-25 | PXD010568 | Pride
2019-03-26 | PXD012515 | Pride
2019-07-03 | PXD014346 | Pride
2019-04-24 | PXD012335 | Pride
2018-05-16 | PXD008689 | Pride