Proteomics

Dataset Information

3

Proteomic analysis of septic renal acute phase response


ABSTRACT: Sepsis-induced acute kidney injury (AKI) is the most common form of AKI with poor outcomes. Renal proteomic analysis after bacterial lipopolysaccharide (LPS) administration revealed that the local renal acute phase reaction (APR) is one of the strongest responses of the kidney during septic AKI in mice. Evaluation of mRNA expression confirmed that most acute phase proteins were produced in the kidney. Our study also provides missing information on the time course of septic renal APR. Proteomic analysis of LPS-induced AKI demonstrated a marked upregulation of local renal acute phase response (APR) that commenced a few hours post injection and peaked at 24 h. Much more APPs were involved in the renal APR than previously identified.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Clostridioides difficile 630   Pseudomonas aeruginosa PAO1   Entamoeba histolytica HM-1:IMSS-A   Entamoeba histolytica   Geobacter sulfurreducens   Beta vulgaris subsp. vulgaris   Helicobacter pylori 26695   Ovis aries   Dentex dentex   Chlorocebus sabaeus   Ensifer adhaerens   Thermoplasma acidophilum DSM 1728   Pyrococcus furiosus   Schizosaccharomyces pombe 927   Medicago truncatula   Pectobacterium carotovorum   Taeniopygia guttata   Sus scrofa domesticus   Bos taurus   Equus caballus   Candida albicans SC5314   Physcomitrium patens   Bursaphelenchus xylophilus   Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10   Synechocystis sp. PCC 6803   Emiliania huxleyi   Drosophila   Callinectes sapidus   Dasypogon diadema   Trypanosoma cruzi   Bacillus subtilis subsp. subtilis str. 168   Ophrys x arachnitiformis subsp. archipelagi   Schizosaccharomyces pombe   Enterococcus faecalis   Trypanosoma brucei brucei   Pseudomonas syringae   Oncorhynchus mykiss   Candida albicans   Helianthus annuus   Cyanophora paradoxa   Mycolicibacterium smegmatis   Populus yunnanensis   Trypanosoma brucei   Paucilactobacillus suebicus   Mus musculus   Ctenopharyngodon idella   Caenorhabditis elegans   Danio rerio   Crithidia fasciculata   Ophrys sphegodes   Solanum lycopersicum   Lingula anatina   Ophrys garganica   Bradyrhizobium diazoefficiens USDA 110   Escherichia coli   Acidithiobacillus ferrooxidans ATCC 53993   Nephila clavipes   Chlamydomonas reinhardtii   Apostichopus japonicus   Triticum aestivum   Craterostigmus tasmanianus   Listeria monocytogenes   Allexis   Ostreococcus tauri   Camelus dromedarius   Vitis vinifera   Saccharomyces cerevisiae   Parasynechococcus marenigrum WH 8102   Zea mays   Macaca mulatta   Histomonas meleagridis   Botrytis cinerea   Human immunodeficiency virus 1   Macaca fascicularis   Ictalurid herpesvirus 1   Acinetobacter baumannii   Cercopithecidae   Staphylococcus sp. 1   Drosophila melanogaster   Oryza sativa Indica Group   Bovine leukemia virus   Rattus norvegicus   Heloderma suspectum suspectum   Homo sapiens subsp. 'Denisova'   Sphingomonas sp. Leaf257   Gallus gallus   Cavia porcellus   Pseudomonas fluorescens SBW25   Sinorhizobium meliloti BL225C   Lotus japonicus   Scolopendra morsitans   Rhizobium sp. Leaf68   Lithobius forficatus   Bombyx mori   Scutigera coleoptrata   Pseudomonas putida KT2440   Streptococcus dysgalactiae subsp. equisimilis SK1249   Rattus rattus   Bradyrhizobium japonicum   Strigamia maritima   Staphylococcus aureus   Aerococcus urinae NBRC 15544 = CCUG 36881   Citrobacter rodentium   Alteromonadales   Canis lupus familiaris   Vitis riparia   Flavobacterium columnare   Globicatella sanguinis   Mycobacterium tuberculosis   albimanus section   Pyricularia oryzae   Streptococcus agalactiae FSL S3-026   Arabidopsis thaliana   Pseudomonas pseudoalcaligenes CECT 5344   Pseudomonas putida BIRD-1   Schizosaccharomyces pombe 972h-   Homo sapiens  

TISSUE(S): Kidney

SUBMITTER: Marko Fonovic  

LAB HEAD: Marko Fonovic

PROVIDER: PXD014664 | Pride | 2020-01-08

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
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11B.raw Raw
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11E.raw Raw
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Publications

The Acute Phase Response Is a Prominent Renal Proteome Change in Sepsis in Mice.

Róka Beáta B   Tod Pál P   Kaucsár Tamás T   Vizovišek Matej M   Vidmar Robert R   Turk Boris B   Fonović Marko M   Szénási Gábor G   Hamar Péter P  

International journal of molecular sciences 20191227 1


(1) Background: Sepsis-induced acute kidney injury (AKI) is the most common form of acute kidney injury (AKI). We studied the temporal profile of the sepsis-induced renal proteome changes. (2) Methods: Male mice were injected intraperitoneally with bacterial lipopolysaccharide (LPS) or saline (control). Renal proteome was studied by LC-MS/MS (ProteomeXchange: PXD014664) at the early phase (EP, 1.5 and 6 h after 40 mg/kg LPS) and the late phase (LP, 24 and 48 h after 10 mg/kg LPS) of LPS-induced  ...[more]

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