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SILAC of HepG2 or HepAD38 cell lines with HBV

ABSTRACT: Hepatitis B Virus constitutes a major threat to global public health by infecting hepatocytes, initiating and driving the progress to end-stage liver disease and liver cancer. Curing treatment for HBV infection is yet unavailable, mainly due to unmet gaps in current understanding of HBV-host interaction. Here, multi-omics interrogations were conducted to generate the first landscape of HBV-induced global changes in host transcriptome, translatome and proteome, which identified multiple translatomic events that HBV orchestrated to remodel host proteostasis networks and afford micro-environments essential for HBV proliferation and persistence.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte, Cell Culture

DISEASE(S): Mixed Disorder As Reaction To Stress

SUBMITTER: Menghuan Zhang

LAB HEAD: Ronggui Hu

PROVIDER: PXD014908 | Pride | 2021-03-06

REPOSITORIES: Pride

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Translatomic profiling reveals novel self-restricting virus-host interactions during HBV infection.

Yuan Shilin S Liao Guanghong G Zhang Menghuan M Zhu Yuanfei Y Wang Kun K Xiao Weidi W Jia Caiwei C Dong Minhui M Sun Na N Walch Axel A Xu Ping P Zhang Jiming J Deng Qiang Q Hu Ronggui R

Journal of hepatology 20210220 1

<h4>Background & aims</h4>HBV remains a global threat to human health. It remains incompletely understood how HBV self-restricts in the host during most adult infections. Thus, we performed multi-omics analyses to systematically interrogate HBV-host interactions and the life cycle of HBV.<h4>Methods</h4>RNA-sequencing and ribosome profiling were conducted with cell-based models for HBV replication and gene expression. The novel translational events or products hereby detected were then character ...[more]

PMID: 33621634

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