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Human glioma cell-line proteomics upon treatment with different drugs

ABSTRACT: Increasing evidence suggests that induction of lethal autophagy carries potential significance for the treatment of glioblastoma (GBM). In continuation of our previous work, we investigated if autophagy-inducing compunds can trigger ATG-dependent cell death in human MZ-54 GBM cells and which pathways may be affected using TMT10-based quantitative proteomics as one of the tools.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain

SUBMITTER: Georg Tascher

LAB HEAD: Christian Münch

PROVIDER: PXD015074 | Pride | 2021-02-26

REPOSITORIES: Pride

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Autophagy activation, lipotoxicity and lysosomal membrane permeabilization synergize to promote pimozide- and loperamide-induced glioma cell death.

Meyer Nina N Henkel Lisa L Linder Benedikt B Zielke Svenja S Tascher Georg G Trautmann Sandra S Geisslinger Gerd G Münch Christian C Fulda Simone S Tegeder Irmgard I Kögel Donat D

Autophagy 20210119 11

Increasing evidence suggests that induction of lethal macroautophagy/autophagy carries potential significance for the treatment of glioblastoma (GBM). In continuation of previous work, we demonstrate that pimozide and loperamide trigger an ATG5- and ATG7 (autophagy related 5 and 7)-dependent type of cell death that is significantly reduced with cathepsin inhibitors and the lipid reactive oxygen species (ROS) scavenger α-tocopherol in MZ-54 GBM cells. Global proteomic analysis after treatment wit ...[more]

PMID: 33461384

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Project description:Autophagy involves massive degradation of intracellular components and functions as a conserved system that helps cells to adapt to adverse conditions. In Arabidopsis thaliana, submergence induces the transcription of autophagy-related (ATG) genes and the formation of autophagosomes. To study the role of autophagy during submergence, we performed transcriptome analysis with atg5, an autophagy-defective mutant, under submergence conditions. Our data showed that submergence changed the expression profile of DEG in the atg5 versus wild-type.

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2014-04-24 | E-GEOD-57031 | biostudies-arrayexpress

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Translational control of autophagy by Orb in the Drosophila germline [early]

2016-07-03 | E-GEOD-74474 | biostudies-arrayexpress

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