Assessing protein sequence database suitability using de novo sequencing

ABSTRACT: The analysis of samples from unsequenced and/or understudied species as well as samples where the proteome is derived from multiple organisms poses two key questions. The first is whether the proteomic data obtained from an unusual sample type even contains peptide tandem mass spectra. The second question is whether an appropriate protein sequence database is available for proteomic searches. We describe the use of automated de novo sequencing for evaluating both the quality of a collection of tandem mass spectra and the suitability of a given protein sequence database for searching that data. Applications of this method include the proteome analysis of closely related species, metaproteomics, and proteomics of extant organisms.

INSTRUMENT(S): Orbitrap Fusion, Q Exactive

ORGANISM(S): Homo Sapiens (human) Glacier Metagenome Caenorhabditis Elegans Ursus Deningeri Hydrolagus Colliei Leucoraja Erinacea Diaphorina Citri (asian Citrus Psyllid) Marine Metagenome

TISSUE(S): Cell Culture, Whole Body, Long Bone

SUBMITTER: Richard Johnson  

LAB HEAD: Michael J. MacCoss

PROVIDER: PXD015083 | Pride | 2019-11-20

REPOSITORIES: Pride